%0 Journal Article
%T Microsatellite cross-species amplification in the genus Centaurea (Compositae)
%A L¨®pez-Vinyallonga
%A S.
%J Collectanea Botanica
%D 2011
%I Consejo Superior de Investigaciones Cient¨ªficas
%X Microsatellite cross-species amplification in the genus Centaurea (Compositae).- Microsatellites are widely used for population genetic studies although the development of these species-specific markers is costly and time-consuming. One strategy for saving time and money is the use of markers developed for one species (source species) in a different species (target species). This is known as cross-amplification. In the present work, two sets of microsatellites are used to test their transferability to six narrow endemic Centaurea species: i) 16 nuclear loci previously published for three congeneric species and ii) 10 universal chloroplast markers designed from Nicotiana tabacum sequences. Seventeen of the 26 markers tested were transferable and 14 of them were also polymorphic and therefore useful for future works. Nuclear markers were more variable and thus more informative than chloroplast markers. Interspecific amplification performed better for the nuclear loci developed for different Centaurea species than for the universal chloroplast markers developed for Nicotiana tabacum. Likewise, transferability was more successful for the species from sect. Phalolepis than for sect. Lepteranthus. Therefore, our results support the idea that the success of the cross-amplification is influenced by the evolutionary distance between the target and the source species. Amplificaci¨®n cruzada de microsat¨¦lites en el g¨¦nero Centaurea (Compositae).- Los microsat¨¦lites se usan habitualmente en estudios de gen¨¦tica de poblaciones aunque su desarrollo es un proceso caro y largo dada su elevada especificidad. Una estrategia que permite ahorrar tiempo y dinero es la llamada amplificaci¨®n cruzada que consiste en amplificar el DNA de una especie determinada (especie objetivo) usando marcadores que han sido dise ados para una especie diferente (especie fuente). En este trabajo se ha realizado un ensayo sobre amplificaci¨®n cruzada usando seis endemismos del g¨¦nero Centaurea y dos conjuntos de microsat¨¦lites: i) 16 marcadores nucleares desarrollados para tres especies congen¨¦ricas y ii) 10 marcadores cloropl¨¢sticos universales dise ados para Nicotiana tabacum. Diecisiete de los 26 marcadores evaluados resultaron transferibles, de los cuales 14 fueron polim¨®rficos siendo as¨ª ¨²tiles para futuros trabajos. Las regiones nucleares se mostraron m¨¢s variables y por lo tanto m¨¢s informativas que las cloropl¨¢sticas. La amplificaci¨®n cruzada funcion¨® mejor para los marcadores nucleares espec¨ªficos de Centaurea que para los cloropl¨¢sticos universales espec¨ªficos de Nicotiana tabacum. A su
%K Chloroplast microsatellites
%K Conservation
%K Cross-amplification
%K Interspecific amplification
%K Narrow endemics
%K SSR
%K Transferability
%K Universal markers
%K Amplificaci¨®n cruzada
%K Amplificaci¨®n interespec¨ªfica
%K Conservaci¨®n
%K Marcadores universales
%K Microendemismos
%K Microsat¨¦lites cloropl¨¢sticos
%K SSR
%U http://collectaneabotanica.revistas.csic.es/index.php/collectaneabotanica/article/view/181/183