%0 Journal Article
%T High genetic diversity among Pseudomonas aeruginosa and Acinetobacter spp. isolated in a public hospital in Brazil
%A Vera L¨²cia Dias Siqueira
%A Rosilene Fressatti Cardoso
%A Rubia Andreia Falleiros de P¨¢dua
%A Katiany Rizzieri Caleffi-Ferracioli
%J Brazilian Journal of Pharmaceutical Sciences
%D 2013
%I Universidade de S?o Paulo
%X In Brazil and other regions of the world, Pseudomonas aeruginosa and Acinetobacter spp. have emerged as important agents of nosocomial infection and are commonly involved in outbreaks. The main objective of the present study was to evaluate the genetic relationship among P. aeruginosa and Acinetobacter spp. isolated from patients in a public university hospital in northwestern Paran¨¢, Brazil, and report their antimicrobial resistance profile. A total of 75 P. aeruginosa and 94 Acinetobacter spp. isolates were phenotypically identified and tested for antibiotic susceptibility using automated methodology. Polymyxin B was tested by disk diffusion for P. aeruginosa. Metallo-¦Â-lactamase (MBL) was detected using a disk approximation test. Genotyping was performed using enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Approximately 55% of the P. aeruginosa isolates and 92% of the Acinetobacter spp. isolates were multiresistant, but none were MBL-producers. ERIC-PCR revealed the presence of small clusters of carbapenem-resistant Acinetobacter spp., most likely OXA-type carbapenemase producers. Furthermore, high genetic diversity in P. aeruginosa and Acinetobacter spp. clinical isolates was observed, suggesting that cross-transmission is not very frequent in the studied hospital. No Brasil, bem como em outras regi es do mundo, Pseudomonas aeruginosa e Acinetobacter spp. surgiram como importantes agentes de infec  o nosocomial e s o comumente envolvidos em surtos. O objetivo principal deste estudo foi descrever a rela  o gen¨¦tica de P. aeruginosa e Acinetobacter spp. isoladas de pacientes internados em hospital universit¨¢rio p¨²blico do noroeste do Paran¨¢ - Brasil e reportar o perfil de resist¨ºncia dessas bact¨¦rias. Um total de 75 P. aeruginosa e 94 Acinetobacter spp. isolados foi fenotipicamente identificado e testado para a suscetibilidade aos antibi¨®ticos por metodologia automatizada. A polimixina B foi testada por difus o em disco para P. aeruginosa. Metalo-¦Â-lactamase (MBL) foi detectada por disco-aproxima  o. An¨¢lise genot¨ªpica foi realizada por enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Aproximadamente 55% dos isolados de P. aeruginosa e 92% de Acinetobacter spp. isolados foram multirresistentes, mas nenhum foi produtor de MBL. Os resultados de ERIC-PCR revelaram pequenos grupamentos de Acinetobacter spp. resistentes aos carbapen¨ºmicos, provavelmente pela produ  o de carbapenemases do tipo OXA. Al¨¦m disso, alta diversidade gen¨¦tica entre os isolados de P. aeruginosa e Acinetobac
%K Pseudomonas aeruginosa
%K Pseudomonas aeruginosa
%K Acinetobacter spp.
%K Acinetobacter spp.
%K Resist¨ºncia antimicrobiana
%K Tipagem bacteriana
%K Pseudomonas aeruginosa
%K Pseudomonas aeruginosa
%K Acinetobacter spp.
%K Acinetobacter spp.
%K Antimicrobial resistance
%K Bacterial typing
%U http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100006