%0 Journal Article
%T Enzymatic scavenging of oxygen dissolved in water: Application of response surface methodology in optimization of conditions
%A Karimi Afzal
%A Mahdizadeh Fatemeh
%A Salari Dariush
%A Vahabzadeh Farzaneh
%J Chemical Industry and Chemical Engineering Quarterly
%D 2012
%I Association of the Chemical Engineers
%R 10.2298/ciceq110823020k
%X In this work, removal of dissolved oxygen in water through reduction by glucose, which was catalyzed by glucose oxidase ¨C catalase enzyme, was studied. Central composite design (CCD) technique was applied to achieve optimum conditions for dissolved oxygen scavenging. Linear, square and interactions between effective parameters were obtained to develop a second order polynomial equation. The adequacy of the obtained model was evaluated by the residual plots, probability-value, coefficient of determination, and FisherˇŻs variance ratio test. Optimum conditions for activity of two enzymes in water deoxygenation were obtained as follows: pH=5.6, T=40ˇăC, initial substrate concentration [S] = 65.5 mmol/L and glucose oxidase activity [E] = 252 U/Lat excess amount of catalase. The deoxygenation process during 30 seconds, in the optimal conditions, was predicted 98.2%. Practical deoxygenation in the predicted conditions was achieved to be 95.20% which was close to the model prediction.
%K central composite design
%K deoxygenation
%K dissolved oxygen
%K oxygen scavenger
%U http://www.doiserbia.nb.rs/img/doi/1451-9372/2012/1451-93721200020K.pdf