%0 Journal Article
%T Molecular Cloning and Functional Characterization of Mouse ¦Á3(IV)NC1
%A Chandra Shekhar Boosani
%A Akulapalli Sudhakar
%J Clinical Medicine : Oncology
%D 2008
%I 
%X Non-collagenous ¦Á3 chain of type IV collagen or ¦Á3(IV)NC1, a 28 kDa C-terminal domain of collagen type IV is a specific inhibitor of endothelial cell translation and angiogenesis. In the present study we have cloned and expressed mouse ¦Á3(IV)NC1 in baculovirus system. The recombinant protein was expressed in soluble form and tested for several of its biological functions. We identified that this recombinant mouse ¦Á3(IV)NC1 specifically inhibited proliferation, translation and tube formation of endothelial cells. Also, we show that ¦Á3(IV)NC1 treatment results in apoptosis specifically in proliferating endothelial cells. In addition we report for the fi rst time that mouse ¦Á3(IV)NC1 inhibits migration and p38 MAPK phosphorylation in addition to inhibition of FAK/Akt/mTOR/4E-BP1 signaling. In mice ¦Á3(IV)NC1 treatment reduced tumor growth and CD-31 positive endothelial vasculature in tumors. Collectively, our data demonstrate the expression of biologically active form of mouse ¦Á3(IV)NC1 in Sf-9 cells and provide important mechanistic insights on ¦Á3(IV)NC1 antiangiogenic actions in endothelial cells.
%K ¦Á3(IV)NC1
%K non-collagenous ¦Á3 chain of type IV collagen
%K AcNPV
%K Autographa californica nuclear polyhedrosis virus
%K Sf-9
%K Spodoptera frugiperda
%K MLEC
%K mouse lung endothelial cells
%K HUVEC
%K human umbilical vein endothelial cells
%K FCS
%K fetal calf serum
%K PMSF
%K Phenylmethylsulfonyl Fluoride
%K TNF-¦Á
%K tumor necrosis factor alpha
%K DEVD
%K cell membrane permeable caspase inhibitor
%K NC
%K negative control
%K PC
%K positive control
%K FN
%K fi bronectin
%K MAPK
%K mitogen activated protein kinase
%K FAK
%K focal
%U http://la-press.com/article.php?article_id=541