%0 Journal Article
%T A Low Cost and Versatile STED Superresolution  Fluorescent Microscope
%A Daniel McBride
%A Chin Su
%A Jun Kameoka
%A Stanislav Vitha
%J Modern Instrumentation
%P 41-48
%@ 2165-9273
%D 2013
%I Scientific Research Publishing
%R 10.4236/mi.2013.23007
%X <p class=\"MsoNormal\">
	<span lang=\"EN-US\">A versatile and inexpensive super-resolution fluorescent microscope that functions as easily as a conventional confocal microscope is described. Components of the microscope were designed on a platform which was placed atop a surplus microscope frame. All optical components and equipment</span><span lang=\"EN-US\">s</span><span lang=\"EN-US\"> used are given. The excitation and depletion beams are ex</span><span lang=\"EN-US\">tracted from a compact low-cost supercontinuum light source. The focal spot of the depletion beam at the focal plane is studied and imaged by a 100 nm aperture near-field fiber tip. The collinear excitation and depletion beam focused by a 0.9 numerical aperture microscope objective produce a 90 nm lateral super-resolution as verified by imaging 100 nm diameter fluorescent beads.</span><span lang=\"EN-US\"><!--?xml:namespace prefix = o /--><o:p></o:p></span> 
</p>
%K Stimulated Emission-Depletion Microscopy
%K STED
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=34456