%0 Journal Article
%T PKC Is a Target to Modulate the Expression of Receptor Mediated Endocytosis (RME)  Mice Macrophages BALB/c for Optimizing the Phagocytosis toward Candida albicans
%A Adi Prayitno
%A Elyana Asnar
%A Okid Parama Astirin
%A Anief Nur Artanti
%A Meutia Srikandi Fitria
%A Eva Agustina Perwitasari
%A Suhartono Taat Putra
%J Journal of Immune Based Therapies, Vaccines and Antimicrobials
%P 44-48
%@ 2168-1554
%D 2013
%I Scientific Research Publishing
%R 10.4236/jibtva.2013.24006
%X <p class=\"MsoNormal\">
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	<b><span lang=\"EN-US\">Introduction:</span></b><span lang=\"EN-US\"> </span><span lang=\"EN-US\">The existence of receptor-mediated endocytosis (RME) means that selectivity and selectivity occurs in capturing macromolecules. Protein kinase C (PKC) which can be expressed by almost all cells are proteins important in signal transduction groove that plays a role in a number of cell activity</span><span lang=\"EN-US\">,</span><span lang=\"EN-US\"><span> e.g. phagocytosis. </span><b><span>Aims:</span></b><span> The purpose of this study </span></span><span lang=\"EN-US\">i</span><span lang=\"EN-US\">s to determine the expression of RME after modulat</span><span lang=\"EN-US\">ing</span><span lang=\"EN-US\"><span> the PKC which is characterized by the number of </span><i><span>Can</span></i></span><i><span lang=\"EN-US\">dida albicans</span></i><span lang=\"EN-US\"><span> cells attached to the surface of macrophages. </span><b><span>Methods:</span></b><span> Peritoneal macrophages cultured BALB/c mice </span></span><span lang=\"EN-US\">are</span><span lang=\"EN-US\"> treated with PMA and</span><span lang=\"EN-US\">/</span><span lang=\"EN-US\">or bisindolylmaleimides of providing levels of 5</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">ng/ml to 100</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\"><span>ng/ml for 10 minutes. Then immediately insert </span><i><span>Candida albicans</span></i><span> and observe every 30 minutes for 120 minutes. </span></span><span lang=\"EN-US\">The </span><span lang=\"EN-US\"><span>research design used the same subject. Data collected in the form of number of </span><i><span>Candida albicans</span></i><span> cells attached to the surface of macrophages </span></span><span lang=\"EN-US\">are</span><span lang=\"EN-US\"><span> analyzed with ANOVA statistical test (one way) to show the differences between treatments. </span><b><span>Results:</span></b><span> The test show</span></span><span lang=\"EN-US\">s</span><span lang=\"EN-US\"><span> statistically significant difference in the number of </span><i><span>Candida albicans</span></i><span> cells attached to the surface of macrophages after administration of various levels of PMA (</span><i><span>p</span></i></span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">&lt;</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">0.001). The higher level of PMA is given, the more active </span><span lang=\"EN-US\">the </span><span lang=\"EN-US\">PKC</span><span lang=\"EN-US\"> is</span><span
%K PMA
%K Bisindolylmaleimides
%K RME
%K PKC
%K Phagocytosis
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=37679