%0 Journal Article
%T Isolation of Aromatic Polyketide Producing Soil Streptomyces Using Combinatorial Screening Strategies
%A Sunita Bundale
%A Deovrat Begde
%A Nandita Nashikkar
%A Tukaram Kadam
%A Avinash Upadhyay
%J Open Access Library PrePrints
%V 1
%N 1
%P 1-16
%@ 2333-9721
%D 2014
%I Open Access Library
%R 10.4236/oalib.preprints.1200010
%X Streptomyces, the soil dwelling filamentous genera of actinomycetes, are known to produce aromatic polyketides, which exhibit antibacterial, antifungal, antiviral and anticancer activities. The purpose of the current study was to screen soil samples for aromatic polyketide producing Streptomyces spp. using a judicious combination of conventional and molecular screening methods. A total of 54 isolates were isolated from soil samples collected from in and around Nagpur, Maharashtra, India. These isolates were characterized by conventional methods. The bioactivity of the isolates was assessed by agar cross streak method against B.subtilis, M.luteus, E.coli, P. aeruginosa, C.albicans and A.niger. Out of the 54 isolates, 31isolates (57%) showed activity against one or other test organism. These 31 bioactive isolates were subjected to secondary screening using agar well diffusion method, whereby 21 isolates were consistent in their bioactivity.  The production of aromatic polyketides by these 21 bioactive isolates was determined by spectroscopic studies and chemical screening of their organic extracts.  11 isolates, which tested positive, were tested for PCR amplification of KS¦Á fragment of type II PKS gene using degenerate primer pair act04/act06.The obtained results showed that 9 isolates (16.66%) showed the ability to produce aromatic polyketides. Out of these 9 isolates, five potent isolates were identified using 16S rRNA gene sequencing and their accession numbers have been deposited in GenBank. The identity of these isolates confirmed their ability to produce aromatic polyketides.  This study indicated that combinatorial screening strategies can be used for correct identification of aromatic polyketide producing culturable Streptomyces spp from soil.
%K Streptomyces
%K aromatic polyketides
%K screening
%K PCR amplification
%U http://www.oalib.com/paper/2996305