%0 Journal Article
%T Synthesis and In Vitro Antitumor Activity of Two Mixed-Ligand Oxovanadium(IV) Complexes of Schiff Base and Phenanthroline
%A Yongli Zhang
%A Xiangsheng Wang
%A Wei Fang
%A Xiaoyan Cai
%A Fujiang Chu
%A Xiangwen Liao
%A Jiazheng Lu
%J Bioinorganic Chemistry and Applications
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/437134
%X Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, 1H NMR, and magnetic susceptibility measurements. Their antitumor effects on BEL-7402, HUH-7, and HepG2 cells were studied by MTT assay. The antitumor biological mechanism of these two complexes was studied in BEL-7402 cells by cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay, and detection of mitochondrial membrane potential (¦¤¦·m). The results showed that the growth of cancer cells was inhibited significantly, and complexes 1 and 2 mainly caused in BEL-7402 cells G0/G1 cell cycle arrest and induced apoptosis. Both 1 and 2 decreased significantly the ¦¤¦·m, causing the depolarization of the mitochondrial membrane. Complex 2 showed greater antitumor efficiency than that of complex 1. 1. Introduction Schiff bases are an important class of ligands because such ligands and their transition metal complexes have a variety of applications including biological, clinical, and analytical applications [1]. The development of the field of bioinorganic chemistry has increased the interest in Schiff base complexes, because it has been recognized that N and S atoms play a key role in the coordination of transition metals at the active sites of many metallobiomolecules [2, 3]. The importance of metal ions in biological systems is well established. One of the most interesting features of metal-coordinated systems is the concerted spatial arrangement of the ligands around the metal ion [3¨C5]. Among the various transition metal ions used in pharmacological studies, Vanadium and its derivatives have been reported to display different biological effects including antitumor, antimicrobial, antihyperlipidemia, antihypertension, antiobesity, enhancement of oxygen affinity of hemoglobin and myoglobin, insulin-enhancing effects, and so on [6¨C8]. Vanadium complexes have also been explored for lowering of glucose levels [9¨C12], diuretic and natriuretic effects, antitumor activity against chemical carcinogenesis in animals and malignant cell lines (in vitro). Much effort has been done for vanadyl species coordinated to organic ligands on the research of their mimetic effects in hopes of developing vanadodrugs [13¨C15]. Because V(IV) complexes have no charge, they are perceived to be candidates for easy bioabsorption. On the other hand,
%U http://www.hindawi.com/journals/bca/2013/437134/