%0 Journal Article
%T Statistical Analysis of Metal Chelating Activity of Centella asiatica and Erythroxylum cuneatum Using Response Surface Methodology
%A R. J. Mohd Salim
%A M. I. Adenan
%A A. Amid
%A M. H. Jauri
%A A. S. Sued
%J Biotechnology Research International
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/137851
%X The purpose of the study is to evaluate the relationship between the extraction parameters and the metal chelating activity of Centella asiatica (CA) and Erythroxylum cuneatum (EC). The response surface methodology was used to optimize the extraction parameters of methanolic extract of CA and EC with respect to the metal chelating activity. For CA, Run 17 gave optimum chelating activity with IC50 = 0.93£¿mg/mL at an extraction temperature of 25¡ãC, speed of agitation at 200£¿rpm, ratio of plant material to solvent at 1£¿g£¿:£¿45£¿mL and extraction time at 1.5 hour. As for EC, Run 13 with 60¡ãC, 200£¿rpm, 1£¿g£¿:£¿35£¿mL and 1 hour had metal chelating activity at IC50 = 0.3817£¿mg/mL. Both optimized extracts were further partitioned using a solvent system to evaluate the fraction responsible for the chelating activity of the plants. The hexane fraction of CA showed potential activity with chelating activity at IC50 = 0.090 and the ethyl acetate fraction of EC had IC50 = 0.120£¿mg/mL. The study showed that the response surface methodology helped to reduce the extraction time, temperature and agitation and subsequently improve the chelating activity of the plants in comparison to the conventional method. 1. Introduction The knowledge and practice of traditional medicine are universal amongst the respected ethnic groups in each country. In Malaysia the benefits of herbal medicine are being conveyed down from one generation to another. Latif et al. [1] state that there are four sources of traditional Malaysian medicine, namely, Malay village medicine (including Orang Asli medicine), Chinese medicine (introduced from China), Indian medicine (introduced from India), and other forms of traditional medicine (including those introduced by the Javanese, Sumatrans, Arabs, Persians, Europeans, etc.). Centella asiatica (CA) also locally known as pegaga is a crawling plant usually growing wildly in a humid climate around the globe. Its wide medicinal benefits include wound healing, enhancing memory, treating mental weariness [2], anti-inflammatory property [3], anticancer activity [4], antilipid peroxidativity [5], and free radical scavenger [6]. Erythroxylum cuneatum forma cuneatum (Miq.) Kurz (EC) is a genus of tropical flowering plants in the family of Erythroxylaceae [7]. While CAs are being well studied for their various medicinal fortunes Erythroxylum cuneatum (EC) on the other hand has a very limited report on its medicinal value. In Terengganu, the leaves are pounded and applied on the forehead of women after miscarriage. In Bunguran, Indonesia leaves are reported to be used
%U http://www.hindawi.com/journals/btri/2013/137851/