%0 Journal Article
%T Antihypercholesterolemic and Antioxidative Potential of an Extract of the Plant, Piper betle, and Its Active Constituent, Eugenol, in Triton WR-1339-Induced Hypercholesterolemia in Experimental Rats
%A Karuppasamy Venkadeswaran
%A Arumugam Ramachandran Muralidharan
%A Thangaraj Annadurai
%A Vasanthakumar Vasantha Ruban
%A Mahalingam Sundararajan
%A Ramalingam Anandhi
%A Philip A. Thomas
%A Pitchairaj Geraldine
%J Evidence-Based Complementary and Alternative Medicine
%D 2014
%I Hindawi Publishing Corporation
%R 10.1155/2014/478973
%X Hypercholesterolemia is a dominant risk factor for atherosclerosis and cardiovascular diseases. In the present study, the putative antihypercholesterolemic and antioxidative properties of an ethanolic extract of Piper betle and of its active constituent, eugenol, were evaluated in experimental hypercholesterolemia induced by a single intraperitoneal injection of Triton WR-1339 (300£¿mg/kg£¿b.wt) in Wistar rats. Saline-treated hypercholesterolemic rats revealed significantly higher mean blood/serum levels of glucose, total cholesterol, triglycerides, low density and very low density lipoprotein cholesterol, and of serum hepatic marker enzymes; in addition, significantly lower mean serum levels of high density lipoprotein cholesterol and significantly lower mean activities of enzymatic antioxidants and nonenzymatic antioxidants were noted in hepatic tissue samples from saline-treated hypercholesterolemic rats, compared to controls. However, in hypercholesterolemic rats receiving the Piper betle extract (500£¿mg/kg£¿b.wt) or eugenol (5£¿mg/kg£¿b.wt) for seven days orally, all these parameters were significantly better than those in saline-treated hypercholesterolemic rats. The hypercholesterolemia-ameliorating effect was better defined in eugenol-treated than in Piper betle extract-treated rats, being as effective as that of the standard lipid-lowering drug, lovastatin (10£¿mg/kg£¿b.wt). These results suggest that eugenol, an active constituent of the Piper betle extract, possesses antihypercholesterolemic and other activities in experimental hypercholesterolemic Wistar rats. 1. Introduction Hypercholesterolemia is a major socioeconomic problem in common individuals as well as health professionals due to the strong correlation between cardiovascular diseases and lipid abnormalities [1]. The modern lifestyle, with a high fat diet and little physical activity, significantly contributes to hypercholesterolemia and cardiovascular diseases [2]. High levels of low-density lipoprotein (LDL) cholesterol accumulate in the extracellular subendothelial space of arteries; these are highly atherogenic and toxic to vascular cells, leading to atherosclerosis, hypertension, obesity, diabetes, and functional depression in organs such as the liver, heart, and kidneys [3]. Clinical trials have shown that lowering lipids reduces the morbidity and mortality associated with cardiovascular complications [4]. Intensive reduction of LDL-cholesterol levels have also been found to reverse atherosclerosis and decrease the progression of cardiovascular disease [5, 6]. Oxidative stress induced
%U http://www.hindawi.com/journals/ecam/2014/478973/