%0 Journal Article
%T Phenolic Composition and Antioxidant and Antimicrobial Activities of Extracts Obtained from Crataegus azarolus L. var. aronia (Willd.) Batt. Ovaries Calli
%A Radhia Bahri-Sahloul
%A Radhia Ben Fredj
%A Naima Boughalleb
%A Jih¨¨ne Shriaa
%A Saad Saguem
%A Jean-Louis Hilbert
%A Francis Trotin
%A Saida Ammar
%A Sadok Bouzid
%A Fethia Harzallah-Skhiri
%J Journal of Botany
%D 2014
%I Hindawi Publishing Corporation
%R 10.1155/2014/623651
%X Objective. Plant cell culture is an innovative technology to produce a variety of substances. Numerous plants synthesize among their secondary metabolites phenolic compounds which possess antioxidant and antimicrobial effects. Hawthorn (Crataegus) is one of these plants which has long been used in folk medicine and is widely utilized in pharmaceutical preparations mainly in neuro- and cardiosedative actions. Methods and Results. The production of polyphenol by fifty-two-week-old Crataegus azarolus var. aronia calli was studied in relation to growth variation and antioxidant and antimicrobial capacity within a subcultured period. The DPPH and ABTS+ assays were used to characterize the antioxidant actions of the callus cultures. Antimicrobial activity was tested by using disc diffusion and dilution assays for the determination of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) values of each active extract. High TEACDPPH, TEACABTS, and antimicrobial activity was observed when maximal growth was reached. An optimum of total phenol, proanthocyanidins, flavonoid, (£¿)-epicatechin, procyanidin B2, chlorogenic acid, and hyperoside was produced during this period. Conclusion. Antioxidant and antimicrobial activities were strongly correlated with total phenols and total flavonoids. Crataegus azarolus var. aronia cells culture represents an important alternative source of natural antioxidants and antimicrobials. 1. Introduction In recent years considerable attention has been devoted to medicinal plants with antioxidant and antimicrobial properties. The antioxidant properties are commonly postulated to play an important role in preventing diseases caused by oxidative stress, such as cancer, coronary arteriosclerosis, and the ageing processes [1]. Phenolic compounds are known to possess different pharmacological activities among which antioxidant and antimicrobial effects have recently received more intention. There is much literature concerning the antioxidant and antimicrobial properties of many species from genus Crataegus L. (hawthorn). The genus Crataegus, known as ¡°Zaarour¡± in Tunisia, is represented by two species in the flora of Tunisia: C. oxyacanthus ssp. monogyna (Jacq.) Rouy and Camus and Crataegus azarolus L. [2]. Crataegus azarolus is represented by two varieties: Crataegus azarolus var. aronia (Willd.) Batt. and C. azarolus var. eu-azarolus Maire; they differ by the color of fruit: yellow fruits for the former and red ones for the latter. The chemistry and pharmacology of hawthorn is well documented [3¨C13].
%U http://www.hindawi.com/journals/jb/2014/623651/