%0 Journal Article
%T Pseudo-MS3 Approach Using Electrospray Mass Spectrometry (ESI-MS/MS) to Characterize Certain (2E)-2-[3-(1H-Imidazol-1-yl)-1-phenylpropylidene]hydrazinecarboxamide Derivatives
%A Ali S. Abdelhameed
%A Adnan A. Kadi
%A Mohamed I. Attia
%A Rihab F. Angawi
%A Mohamed W. Attwa
%A Hany W. Darwish
%J Journal of Chemistry
%D 2014
%I Hindawi Publishing Corporation
%R 10.1155/2014/386301
%X An approach for the use of in-source fragmentation with electrospray ionization followed by product ion scan in a triple quadrupole mass spectrometer system is described. This approach is based on the elucidation of the various fragmentation pathways by further dissociation of each fragment ion in the ion spectrum. This can be achieved predominately, by combining fragmentor voltage induced dissociation (in-source fragmentation) with subsequent collision-induced dissociation; this process can be referred to as pseudo-MS3 scan mode. This technique permitted unambiguous assignment and provided sufficient sensitivity and specificity. It is advantageous for structure elucidation of unknown compounds. We investigate the possibility of using in-source fragmentation with the diverse novel chemical entities encompassing different substituents. This process was intended to improve the qualitative capability of tandem mass spectrometry simulating the MS3 of ion trap for studying fragmentation mechanisms. The approach is to implement the investigated technique as a well established tool for the characterization of new pharmacologically important chemical entities. The data presented in this paper provided useful information on the effect of different substituents on the ionization/fragmentation processes and can be used in the characterization of (2E)-2-[3-(1H-imidazol-1-yl)-1-phenylpropylidene]-hydrazinecarboxamide derivatives 3a¨Ch. 1. Introduction It has always been known that the use of a triple quadrupole mass spectrometer system (QqQ) for quantitative analysis is superior to that of any ion trap mass spectrometry systems. This is due to the fact that one can always utilize the collision-induced dissociation (CID) in the QqQ which usually produces more abundant fragment ions than those shown in case of resonance excitation in the ion trap (IT) [1]. However, the opposite has always remained correct with regard to qualitative analysis. On the other hand, electrospray ionization mass spectrometry (ESI-MS)¡ªas a robust technique for quantification studies¡ªhas been excessively applied for the analysis of different types of compounds such as oligosaccharides, glycoproteins, oligonucleotides, and drug metabolites [2¨C7]. In electrospray ionization (ESI), ions fragmentation may take place inside the ion source (in-source fragmentation) prior to reaching the mass analyzer. Although this method of in-source fragmentation has low specificity, as most of the ions in the ion source are fragmented simultaneously, it has been reported by different scientists [8¨C17]. Elaborate
%U http://www.hindawi.com/journals/jchem/2014/386301/