%0 Journal Article
%T Evaluation of the Antitumor Activity by Ni Nanoparticles with Verbascoside
%A Mingyue Chen
%A Yaqin Zhang
%A Bin Huang
%A Xueming Yang
%A Yunong Wu
%A Bin Liu
%A Yi Yuan
%A Gen Zhang
%J Journal of Nanomaterials
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/623497
%X Verbascoside (VB) has attracted a great deal of attention due to ITS pharmacological properties. In our study, we synthesized a multifunctional verbascoside coated Ni nanoparticles (VB-Ni). Transmission electron microscopy (TEM) and high performance liquid chromatography (HPLC) display the characteristics of VB-Ni nanoparticles. Compared with VB, VB-Ni has been proven to induce apoptosis and resist the growth of doxorubicin-resistant K562 cells in vitro and in vivo. Thus, VB-Ni nanoparticles can be thought of as an ideal mode of cancer treatment. 1. Introduction Cancer is quickly becoming the leading cause of death worldwide [1]. Nickel nanoparticles (Ni NPs) have been applied in a wide range of fields due to their unique structure and properties [2¨C6]. Over the past decades, nanoparticles have been increasingly applied in clinical diagnoses and cancer therapy with promising and far-ranging prospects in the medical fields. Increasing interest in the application of nanotechnology for cancer therapy has been noted [7¨C10]. Previous phytochemical studies have demonstrated that flavonoids and phenylpropanoid glycosides are major bioactive constituents of the Tsoong herb (Chinese name: Banchunmaxianhao, BCM) [11]. Among these constituents, VB has attracted a great deal of attention due to its pharmacological properties [12¨C17]. Its properties include hepatoprotective, anti-inflammatory, antitumor, cytotoxic, and antioxidant activities [18¨C20]. In recent years, many studies on the therapeutic effect of drug-loaded nanoparticles have become a hot spot [21, 22]. Based on the above considerations, we have verified the biological effects of VB-Ni nanoparticles on treating cancer cells [23, 24]. These observations indicate their great potential in clinical and biomedical applications. 2. Materials and Methods 2.1. Materials BCM were collected from Gangcha, QingHai, China, and identified by Professor Li-Juan Mei (Northwest Institute of Plateau Biology, Chinese Academy of Sciences). Materials used for HPLC analysis were of analytical grade. 2.2. Cell Culture K562 cells were purchased from Tianjin Institute of Hematology and cultured in Dulbecco¡¯s Modification of Eagle¡¯s Medium (DMEM) supplemented with 10% FBS (GIBCO) and penicillin (100£¿U/mL)/streptomycin (100£¿mg/mL) at 37¡ãC in a 5% CO2, water-saturated atmosphere. To test the function of VB-Ni, VB-Ni, or VB was added to K562 cells in the same concentration. Cells were observed by microscope after 48 or 72£¿h treatment, using DNA Ladder to detect the apoptosis of cells. 2.3. Extract VB from BCM Plant BCM (500£¿g) were
%U http://www.hindawi.com/journals/jnm/2013/623497/