%0 Journal Article
%T Dried Human Amniotic Membrane Does Not Alleviate Inflammation and Fibrosis in Experimental Strabismus Surgery
%A Bo Young Chun
%A Hong Kyun Kim
%A Jae Pil Shin
%J Journal of Ophthalmology
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/369126
%X Purpose. The purpose of this study was to evaluate the efficacy of dried human amniotic membrane (AM) in reducing the postoperative inflammatory response and scarring after strabismus surgery. Methods. The inflammatory response at the extraocular muscle reattachment site was analyzed after superior rectus (SR) resection in 12 rabbits. Dried human AM (Ambiodry2) was applied between the resected SR muscle plane and TenonĄ¯s capsule of the left eyes of rabbits. As a control, the right eyes of rabbits underwent SR resection only. The surgeon randomly ordered which eye gets operated first during the experiment. Two weeks later, enucleation was performed. Six sagittal sections were made for each eye at the insertion of the SR muscle. The grade of postoperative inflammation and the presence of fibrosis were evaluated in histological examinations. Results. There was no statistically significant difference in the intensity of inflammation and fibrous proliferation between the eyes treated with dried human AM after SR resection and those treated with SR resection only. Conclusions. The use of dried human AM was not effective in controlling the postoperative inflammation and scarring in rabbit eyes after extraocular muscle surgery. However, this may be due to the devitalized dry preparation of human AM (Ambiodry2), which may have lost the expected anti-inflammatory and anti-scarring properties, and further studies on humans may be necessary. 1. Introduction Postoperative adhesion is one of the major complications of strabismus surgery that can cause motility problems that affect the surgical outcomes [1, 2]. Due to the fact that the suppression of inflammation is a key element in the prevention of further fibrovascular proliferation and scar formation in the conjunctiva [3], various approaches have been used to reduce postoperative inflammation and scarring following strabismus surgery. Among these are mechanical barrier devices that isolate the muscle from the sclera and the TenonĄ¯s capsule, steroids, antiproliferative agents and viscoelastic substances [1¨C9]. However, none of these techniques has been widely accepted because of associated complications and inconsistent results. The use of the amniotic membrane (AM) in other applications of ophthalmology such as ocular surface reconstruction, filtration surgery, and the treatment of pterygium has increased [3, 10¨C12]. The AM can promote epithelialization of the cornea and the conjunctiva and reduce inflammation, scarring, and neovascularization in anterior segment surgeries [13, 14]. AM exhibits several
%U http://www.hindawi.com/journals/joph/2013/369126/