%0 Journal Article
%T A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents
%A Lihong Zhang
%A Xiaojin Xiao
%A Zhenzhen Yang
%A Mengli Jiang
%A Xiaodong Li
%J Journal of Spectroscopy
%D 2013
%R 10.1155/2013/919023
%X To bridge the convergence between traditional Chinese medicine (TCM) and modern medicine originated from the West, a new method of area under the absorbance-wavelength curve (AUAWC) by spectrophotometer scanning was investigated and compared with HPLC method to explore metabolomic pharmacokinetics in rats. AUAWC and drug total concentration were obtained after Yangxue was injected to rats. Meanwhile, individual concentrations of sodium ferulate, tetramethylpyrazine hydrochloride, tanshinol sodium, and sodium tanshinone IIA sulfonate in plasma were determined by HPLC. Metabolomic profile of multicomponents plasma concentration time from AUAWC and that of individual components from HPLC were compared. The data from AUAWC had one-compartment model with mean area under concentration versus time (AUC) of 9370.58£¿min ¦̀g/mL and mean elimination half-life ( ) of 12.92£¿min. The results by HPLC demonstrated that sodium ferulate and tetramethylpyrazine hydrochloride had one-compartment model with AUC of 6075.50 and 876.94£¿min ¦̀g/mL, of 10.85 and 20.57£¿min, respectively. Tanshinol sodium and sodium tanshinone IIA sulfonate showed two-compartment model, and AUC was 29.58 and 201.46 with of 1.76 and 16.90, respectively. The profiles indicated that method of AUAWC can be used to study pharmacokinetics of TCM with multicomponents and to improve its development of active theory and application in clinic combined with in vivo metabolomic profile of HPLC. 1. Introduction For around 200 years, two very different systems of medicine have been used in Asia to cure diseases and keep people healthy. The local Asian one is based on traditional Chinese medicine (TCM)¡ªherbal mixtures developed through observation and experience accumulated over thousands of years in China, but with unknown mechanisms of action [1]. On the other hand, modern medicine, which originated from the West, consists of chemically purified compounds that have been discovered through scientific investigation and tested in controlled clinical trials. These two phenomena confirmed the right saying in the career of traditional Chinese medicine that individual drug has its characteristic merits with good effectiveness, but compound formula has excellent clinical application with mixtures reasonable [2]. However, the most classical method, especially high performance liquid chromatography (HPLC), of metabolomic pharmacokinetics studies for western chemical drugs is effectively used to determine the drug metabolomic concentration in blood at different kinetic time [3¨C6]. It can explain the variation of the
%U http://www.hindawi.com/journals/jspec/2013/919023/