%0 Journal Article
%T Specific Metabolic Fingerprint of a Dietary Exposure to a Very Low Dose of Endosulfan
%A C¨¦cile Canlet
%A Marie Tremblay-Franco
%A Roselyne Gautier
%A J¨¦r£¿me Molina
%A Benjamin M¨¦tais
%A Florence Blas-Y Estrada
%A Laurence Gamet-Payrastre
%J Journal of Toxicology
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/545802
%X Like other persistent organochlorine pesticides, endosulfan residues have been detected in foods including fruit, vegetables, and fish. The aim of our study was to assess the impact of a dietary exposure to low doses of endosulfan from foetal development until adult age on metabolic homeostasis in mice and to identify biomarkers of exposure using an 1H-NMR-based metabonomic approach in various tissues and biofluids. We report in both genders an increase in plasma glucose as well as changes in levels of factors involved in the regulation of liver oxidative stress, confirming the prooxidant activities of this compound. Some metabolic changes were distinct in males and females. For example in plasma, a decrease in lipid LDL and choline content was only observed in female. Lactate levels in males were significantly increased. In conclusion, our results show that metabolic changes in liver could be linked to the onset of pathologies like diabetes and insulin resistance. Moreover from our results it appears that the NMR-based metabonomic approach could be useful for the characterization in plasma of a dietary exposure to low dose of pesticide in human. 1. Introduction Many epidemiological studies have shown that exposure to pesticides is a risk factor for human health, as evidenced by the positive correlation between professional exposure to these compounds and an increase in the incidence of various human diseases (reviewed in Merhi et al. [1]). The general population is also exposed to pesticides mainly via food intake. Thus many people have a lifelong exposure to low doses of pesticides, the impact of which on human health is not yet known. Organochlorine (OC) pesticides are among the most frequent contaminants found in the environmental compartments because they persist in the environment and bioaccumulate in organisms, partly due to their lipophilic properties [2]. Endosulfan is a chlorinated cyclodiene pesticide which was first severely restricted then banned in 2006 in several European Union countries. Nevertheless, the general population continues to be exposed: like other persistent organochlorine pesticides, endosulfan residues have been detected in several foods including fruits, vegetables, and fish [3]. There is an evidence that endosulfan is acutely poisonous to humans through both accidental and intentional exposure [4]. Endosulfan is classified by the World Health Organisation as a moderately hazardous (class II) pesticide [5]; however, it is genotoxic [6] and is an endocrine disrupter displaying xenooestrogenic activity [7]. Endosulfan has
%U http://www.hindawi.com/journals/jt/2013/545802/