%0 Journal Article
%T Molecular Detection and Genotyping of Chlamydia psittaci in Captive Psittacines from Costa Rica
%A Jessica Sheleby-El¨ªas
%A ¨¢ntony Sol¨®rzano-Morales
%A Juan Jos¨¦ Romero-Zu£¿iga
%A Gaby Dolz
%J Veterinary Medicine International
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/142962
%X Oropharyngeal and cloacal swabs from 117 captive psittacine birds presented at veterinary clinics (88) and from shelters/rescue centers of wildlife (29) were collected to determine the prevalence of C. psittaci in captive birds in Costa Rica. Samples were collected during 2009 from a total of 19 different species of parrots, with Ara macao (33), Amazona autumnalis (24), Amazona ochrocephala (21), and Ara ararauna (8) being the most representative species sampled. C. psittaci was detected in four (3.4%) birds using molecular detection (PCR). The positive samples belonged to birds presented at veterinary clinics; three of them were Ara macao and one Amazona ochrocephala. Three birds were adults; all positive birds showed no symptoms of illness and lived in homes with other birds, two in San Jos¨¦ and two in Heredia. Sequencing was used to confirm the PCR positive results, showing that two samples of C. psittaci belonged to genotype A, representing the first report of the presence of this genotype in Costa Rica. The detection of this bacterium in captive psittacine birds shows that there is a potential risk for people living or having contact with them and that there is a possibility of infecting other birds. 1. Introduction Avian chlamydiosis is caused by Chlamydia psittaci, a Gram-negative, intracellular bacterium, with nine known genotypes (A¨CF, E/B, M56, and WC) [1]. C. psittaci has been detected in 465 species of birds [2], but the highest infection rates are found in parrots (Psittacidae) and pigeons (Columbiformes). In parrots, the prevalence varies between 16% and 81% [3¨C5]. Progression to clinical infection is dependent on the nature of the infecting strain and on host species. Avirulent strains generally produce asymptomatic infections in adult birds, and these may excrete the organism for several months. Large quantities of the agent can be found regularly or intermittently in feces, lacrimal fluids, nasal discharges, oropharyngeal mucus, and crop milk of infected birds [6]. Prolonged and subacute clinical forms are common. Extreme environmental changes or concurrent infections may cause the onset of clinical disease. Avian chlamydiosis presents from nonspecific clinical signs to acute systemic illness, latter especially in young animals; lethargy, anorexia, dehydration, depression, hyperthermia, nasal and ocular discharges, abnormal excretions, and greenish diarrhea are mainly reported [6, 7]. All C. psittaci genotypes can be transmitted to humans where they also cause a disease called psittacosis or parrot fever [7]. This transmission may occur
%U http://www.hindawi.com/journals/vmi/2013/142962/