%0 Journal Article
%T Characterization of the Fungal Microbiome (Mycobiome) in Fecal Samples from Dogs
%A M. Lauren Foster
%A Scot E. Dowd
%A Christine Stephenson
%A J£¿rg M. Steiner
%A Jan S. Suchodolski
%J Veterinary Medicine International
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/658373
%X The prevalence and phylogenetic description of fungal organisms and their role as part of the intestinal ecosystem have not yet been studied extensively in dogs. This study evaluated the fungal microbiome of 19 dogs (12 healthy dogs and 7 dogs with acute diarrhea) using fungal tag-encoded FLX-Titanium amplicon pyrosequencing. Five distinct fungal phyla were identified, with Ascomycota (medians: 97.9% of obtained sequences in healthy dogs and 98.2% in diseased dogs) and Basidiomycota (median 1.0% in healthy dogs and median 0.5% in diseased dogs) being the most abundant fungal phyla. A total of 219 fungal genera were identified across all 19 dogs with a median (range) of 28 (4¨C69) genera per sample. Candida was the most abundant genus found in both the diseased dogs (median: 1.9%, range: 0.2%¨C38.5% of sequences) and healthy dogs (median: 5.2%, range: 0.0%¨C63.1% of sequences). Candida natalensis was the most frequently identified species. No significant differences were observed in the relative proportions of fungal communities between healthy and diseased dogs. In conclusion, fecal samples of healthy dogs and dogs with acute diarrhea harbor various fungal genera, and their role in gastrointestinal health and disease warrants further studies. 1. Introduction Recent molecular-phylogenetic studies have revealed diverse microbial communities in the canine gastrointestinal (GI) tract and have emphasized the importance of the intestinal microbiota for gastrointestinal health [1]. The intestinal microbiota plays a vital role in the health of the GI tract, participates in the development of the host immune system, and also provides protection from invading pathogens [2]. Several studies have characterized the bacterial communities in the canine GI tract in health and disease [3¨C5]. However, limited information is available about the prevalence and classification of other members of the intestinal microbiome, such as fungal organisms. Previous studies that have described fungal organisms in the canine GI tract have either used culture based methods [6, 7], have utilized molecular-phylogenetic methods on pooled intestinal samples [8, 9], or have analyzed only a limited number of fungal sequences [10]. Studies in humans have suggested that the fungal microbiome may play a role in chronic GI disorders [11, 12]. Therefore, a more detailed description of the fungal microbiome (mycobiome) is needed to better understand the role of fungi in the GI tract of healthy dogs and dogs with GI disease. The aim of this study was to describe the fungal communities present in fecal
%U http://www.hindawi.com/journals/vmi/2013/658373/