%0 Journal Article
%T Primary Corneal Squamous Cell Carcinoma in a Dog: Clinical and Histopathological Evaluation
%A Giovanni Barsotti
%A Lorenzo Ressel
%A Riccardo Finotello
%A Veronica Marchetti
%A Francesca Millanta
%J Case Reports in Veterinary Medicine
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/596593
%X An 8-year-old male pug with a 12-month history of a progressive nonpainful mass on the left cornea was evaluated. Ocular examination showed a severe bilateral keratoconjunctivitis sicca, pigmentary keratitis, and an exophytic irregular pink mass occupying approximately 75% of the total corneal surface of the left eye. A squamous cell carcinoma (SCC) was suspected on cytology, and clinical investigations showed no evidence of metastases. A transpalpebral enucleation was therefore performed, and the diagnosis of SCC was confirmed on histopathology. Immunohistochemical investigations showed that the neoplastic cells were pan-cytokeratin positive and vimentin negative. Additionally, nuclei immunoreactive to Ki-67 antigen were detected. Tumor cells were also negative to p53. Immunoreactivity to COX-2 was found in less than 10% of the neoplastic cells. No adjuvant therapies were instituted, and no evidence of local recurrence or distance metastasis was identified during the 24-month follow-up period. 1. Introduction Neoplasms of the cornea occur uncommonly in dogs although various primary and secondary tumors have been described in veterinary literature [1, 2]. Corneal squamous cell carcinoma (SCC) is considered rare in the dog and often represents a secondary extension of a primary limbal or conjunctival neoplasia [1, 3, 4]. A number of cases of canine primary SCC of the cornea have been described especially in recent years [5¨C10], and some authors report an increased occurrence of the tumor in this period [11]. Only three canine SCCs have been characterized by the use of immunohistochemistry, and p53 protein was generally investigated with varying results [7, 9]. Cyclooxygenase (COX) overexpression has been identified in various neoplastic tissues in humans [12¨C18] and domestic animals [19¨C26]. COX-2 has been found to be strongly expressed in all the cases of canine SCC of the cutis [27]. Since the etiopathogenesis of ocular SCC is still unclear, some authors have evaluated the expression of COX, especially COX-2, in corneal neoplastic tissues of horses and have suggested a possible role of the enzyme in oncogenesis and/or progression of this type of corneal tumor [23, 24]. The aim of the present study was to describe the clinical and histopathological appearance of a primary corneal SCC in an 8 year-old male pug dog, to report its histopathological findings, and to characterise the tumour using antivimentin and antipan cytokeratin antibodies and evaluate the expression of cyclooxygenase-2, p53 protein, and Ki-67 antigen in the neoplastic cornea by the use
%U http://www.hindawi.com/journals/crivem/2012/596593/