%0 Journal Article
%T Oral Lichen Planus in Thai Patients Has a Low Prevalence of Human Papillomavirus
%A Pratanporn Arirachakaran
%A Jira Chansaengroj
%A Woradee Lurchachaiwong
%A Patnarin Kanjanabud
%A Kobkan Thongprasom
%A Yong Poovorawan
%J ISRN Dentistry
%D 2013
%R 10.1155/2013/362750
%X Background. Oral lichen planus (OLP) is a common chronic inflammatory immune-mediated disease, with an etiopathogenesis associated with cell-mediated immunological dysfunction. Viral infection has been hypothesized as a predisposing factor in the pathogenesis of this disease. Viruses may alter host cell function by inducing the abnormal expression of cellular proteins leading to disease development. However, reports on the relationship between human papillomavirus (HPV) and OLP are inconclusive. Objective. To explore the association between HPV and OLP in Thai patients. Materials and Methods. DNA was extracted from thirty-seven fresh-frozen tissue biopsy specimens from OLP lesions, and polymerase chain reaction assay for the L1 and E1 genes covering 32 types of high- and low-risk HPV was performed. Results. HPV DNA was detected in one tissue biopsy from an atrophic-type OLP lesion. All control samples were negative. Genomic sequencing of the E1 gene PCR product demonstrated that the HPV-type 16 found in the lesion is closely related to the East Asian type. Conclusion. Our data indicate a low prevalence of HPV infection in OLP lesions in Thai patients. 1. Introduction Oral lichen planus (OLP) is a chronic inflammatory disease in which the immunopathogenesis involves cell-mediated immune dysregulation [1]. OLP affects 0.5¨C2.2% of the global population. However, prevalence varies according to geographic location [2]. This disease is commonly seen in Thai patients [3], with presentations ranging from symptom-free to a burning sensation or severe pain interfering with phonation, mastication, and deglutition [1]. OLP is classified as a potentially malignant disorder (PMD) of the oral mucosa with a transforming rate of 0¨C6.25% [1, 4]. Histologically, lesions are characterized by hyperkeratosis, basal layer liquefaction of the oral epithelium-connective tissue interface, and a dense infiltration of a band of lymphocytes [5, 6]. Unidentified antigen presented to lymphocytic cells may play a role in this disease. Previous studies suggest that OLP is a T-cell-mediated inflammatory disease in which autocytotoxic CD8+T cells trigger apoptosis in oral epithelial cells [1]. Exogenous agents may also alter keratinocyte antigen expression. The response of these specific CD8+T cells is similar to what occurs during a viral infection where a virus can act as a cytoplasmic antigen or induce the expression of host cell proteins, resulting in an altered host cell protein profile [7]. Therefore, it is of interest to investigate the possibility of viral involvement in the
%U http://www.hindawi.com/journals/isrn.dentistry/2013/362750/