%0 Journal Article
%T No Association between Glycemia and Wound Healing in an Experimental db/db Mouse Model
%A Margrete Berdal
%A Trond Jenssen
%J ISRN Endocrinology
%D 2013
%R 10.1155/2013/307925
%X Impaired wound healing is a frequent problem in diabetes. Hyperglycemia may be an operative mechanism, but a link between glycemic control and wound healing has never been established. Wounds in db/db mice have been extensively studied. This study was undertaken to see if plasma glucose was a predictor of wound healing. An excisional wound was made (149 db/db mice). Wound closure was studied versus metabolic variables. The animals were weeks (mean ¡À standard error of the mean), obese ( £¿g), and hyperglycemic (fasting plasma glucose £¿mmol/L). Wound closure at day 13 was . In linear mixed model analyses neither fasting plasma glucose nor its change from start to end of experiment was a significant predictor of wound closure ( , , 95% CI: £¿0.01 to 0.31 and , , 95% CI: £¿0.11 to 0.23, resp.). However, increase in body weight significantly and independently predicted wound closure (for weight change, , , 95% CI: 0.06 to 0.38). This study strongly suggests that wound healing in db/db mice is independent of prevailing glycemia but dependent on anabolic changes such as weight gain over time. 1. Introduction Impaired wound healing¡ªa well-known problem in diabetes¡ªhas been extensively studied in animals [1, 2]. One model, which has been explored for this purpose, is the genetically leptin receptor deficient, diabetic db/db mouse with characteristics such as obesity, transient hyperinsulinemia, insulin resistance, severe hyperglycemia, and impaired wound healing [2¨C8]. Hyperglycemia is one factor that may be implicated in impaired wound repair. Clinical guidelines advocate optimization of metabolic control to facilitate wound healing [9]. A retrospective study in humans suggested that glycated hemoglobin (A1C) predicted healing rates in diabetic wounds [10]. However, as far as we know, direct evidence of a link between glycemic control and healing is lacking [11]. In the db/db mouse model, a few studies have addressed the possible impact of hyperglycemia on wound healing [6, 12, 13]. They included up to 31£¿db/db mice from one or two age groups, and baseline body weight but not weight change was assessed. No significant associations between metabolic parameters and wound healing were observed [6]. In the present study we included 149£¿db/db mice, aged 6¨C16 weeks, with the aim of testing whether fasting plasma glucose at baseline, the change in fasting plasma glucose, or other metabolic parameters, including weight change, were associated with wound closure. 2. Materials and Methods 2.1. Animals Diabetic C57BL/KsBom-db/db mice were studied. All animals were purchased
%U http://www.hindawi.com/journals/isrn.endocrinology/2013/307925/