%0 Journal Article
%T Plasma Disposition of Conventional and Long-Acting Moxifloxacin in Sheep after Intravenous Administration
%A C. M. Modi
%A S. K. Mody
%A F. D. Modi
%A H. B. Patel
%J ISRN Veterinary Science
%D 2012
%R 10.5402/2012/951306
%X This study describes disposition of long-acting moxifloxacin and conventional formulations of moxifloxacin in sheep after intravenous administration in five male sheep. Long acting moxifloxacin solution (10% moxifloxacin in solution with L-arginine, N-butyl alcohol, and benzyl alcohol) and conventional moxifloxacin (10%) were injected in jugular vein. Blood samples were collected from contralateral jugular vein in test tubes containing 30¨C50£¿IU heparin (anticoagulant) periodically from 0.083 to 72£¿h of drug administration. Drug concentrations in plasma were determined using High-Performance Liquid Chromatography (HPLC) with fluorescence detector. The mobile phase consisted of a mixture of buffer (10£¿gm of tetrabutyl ammonium hydrogen sulphate per liter-deionised water) and acetonitrile (80£¿:£¿20). The buffer was 0.067M of disodium hydrogen phosphate with pH of 7.5. The flow rate was 1£¿mL¡¤min£¿1 at ambient temperature. The effluent was monitored at 296£¿nm excitation and 504£¿nm emissions wavelength. HPLC with fluorescence detector method for plasma moxifloxacin assay was standardized with specific modification for plasma of sheep in the present study. After single-dose intravenous administration of long acting moxifloxacin the plasma concentration of 0.016 ¡À 0.001£¿¦Ìg¡¤mL£¿1 was maintained for up to 72£¿h. Conventional formulation of moxifloxacin remained in body for up to 24£¿h of drug administration with the level of 0.015 ¡À 0.005 ¦Ìg¡¤mL£¿1. 1. Introduction Moxifloxacin is a new 8-methoxy-quinolone with a broad spectrum of antibacterial activity against organisms Gram-positive and Gram-negative bacteria, anaerobes, and atypical organism such as Mycoplasma and Chlamydia spp. It has some characteristics such as a wide spectrum of bactericidal activity, a large volume of distribution, low plasma protein binding, and relatively low Minimal Inhibitory Concentrations (MICs) against susceptible target microorganisms [1, 2], The MIC of moxifloxacin for Mycobacterium ulcerans ranged from 0.015¨C0.5£¿¦Ìg £¿ mL£¿1 [3] and for S. aureus from 0.03¨C0.12£¿¦Ìg £¿ mL£¿1 [4]. It has the highest potency in its class against Staphylococcus aureus and Staphylococcus epidermidis [5]. All these characteristics have made the moxifloxacin a drug of choice to treat many infectious diseases which could not be treated with older fluoroquinolones and other antimicrobials. The disposition study gives concentration versus time profile of drug which is prime step to generate pharmacokinetics data and subsequently to establish dosage regimens. The comparative disposition of long acting and conventional
%U http://www.hindawi.com/journals/isrn.veterinary.science/2012/951306/