%0 Journal Article
%T Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni
%A Venkata Sai Prakash Chaturvedula
%A Steven Chen
%A Oliver Yu
%A Guohong Mao
%J Biomolecules
%D 2013
%I MDPI AG
%R 10.3390/biom3040733
%X From the commercial extract of the leaves of Stevia rebaudiana Bertoni, a minor steviol glycoside, 13-[(2- O-¦Â-D-glucopyranosyl-3- O-¦Â-D-glucopyranosyl-¦Â-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[(2- O-(3- O-¦Â-D-glucopyranosyl-¦Á-L-rhamnopyranosyl)-3- O-¦Â-D-glucopyranosyl-¦Â-D-glucopyranosyl) ester] ( 1); also known as rebaudioside O having seven sugar units has been isolated. Its structural characterization has been achieved by the extensive 1D ( 1H and 13C), and 2D NMR (COSY, HMQC, HMBC) as well as mass spectral data. Further, hydrolysis studies were performed on rebaudioside O using acid and enzymatic methods to identify aglycone and sugar residues in its structure as well as their configurations.
%K Stevia rebaudiana
%K diterpene glycoside
%K isolation
%K structure elucidation
%K spectral data
%K hydrolysis studies
%U http://www.mdpi.com/2218-273X/3/4/733