%0 Journal Article
%T Biochemical Characterization of Prion Strains in Bank Voles
%A Laura Pirisinu
%A Stefano Marcon
%A Michele Angelo Di Bari
%A Claudia D'Agostino
%A Umberto Agrimi
%A Romolo Nonno
%J Pathogens
%D 2013
%I MDPI AG
%R 10.3390/pathogens2030446
%X Prions exist as different strains exhibiting distinct disease phenotypes. Currently, the identification of prion strains is still based on biological strain typing in rodents. However, it has been shown that prion strains may be associated with distinct PrP Sc biochemical types. Taking advantage of the availability of several prion strains adapted to a novel rodent model, the bank vole, we investigated if any prion strain was actually associated with distinctive PrP Sc biochemical characteristics and if it was possible to univocally identify strains through PrP Sc biochemical phenotypes. We selected six different vole-adapted strains (three human-derived and three animal-derived) and analyzed PrP Sc from individual voles by epitope mapping of protease resistant core of PrP Sc (PrP res) and by conformational stability and solubility assay. Overall, we discriminated five out of six prion strains, while two different scrapie strains showed identical PrP Sc types. Our results suggest that the biochemical strain typing approach here proposed was highly discriminative, although by itself it did not allow us to identify all prion strains analyzed.
%K prion
%K prion strain
%K TSE
%K PrPSc
%K bank vole
%K CJD
%K scrapie
%K BSE
%K conformational stability
%K GdnHCl
%U http://www.mdpi.com/2076-0817/2/3/446