%0 Journal Article
%T Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds
%A Hisahiro Kai
%A Ena Akamatsu
%A Eri Torii
%A Hiroko Kodama
%A Chizuko Yukizaki
%A Isao Akagi
%A Hisatoshi Ino
%A Yoichi Sakakibara
%A Masahito Suiko
%A Ikuo Yamamoto
%A Akihiko Okayama
%A Kazuhiro Morishita
%A Hiroaki Kataoka
%A Koji Matsuno
%J Plants
%D 2014
%I MDPI AG
%R 10.3390/plants3010018
%X In our previous report, an 80% ethanol bitter gourd seed extract (BGSE) was found to suppress proliferation of adult T-cell leukemia (ATL) cell lines. The present study aimed to identify the bioactive compounds from BGSE specific against ATL. From the result of an HPLC-MS analysis, ¦Á-eleostearic acid (¦Á-ESA) was present in BGSE at 0.68% ¡À 0.0022% (¡ÀSD, n = 5). In the cell proliferation test, ¦Á-ESA potently suppressed proliferation of two ATL cell lines (ED and Su9T01; IC 50 = 8.9 and 29.3 ¦ÌM, respectively) more than several other octadecanoic acids. However, ¦Á-ESA moderately inhibited phytohemagglutinin-activated human peripheral blood mononuclear cells (PBMC; IC 50 = 31.0 ¦ÌM). These results suggest that BGSE-derived ¦Á-ESA has potential as a functional food constituent because of its activity against ATL, particularly against ED cells. Moreover, ¦Á-ESA might be effective for the prevention of moderate adverse effects of ATL on normal T cells.
%K adult T-cell leukemia
%K bitter gourd seed extract
%K ¦Á-eleostearic acid
%K phytohemagglutinin-activated human peripheral blood mononuclear cell
%U http://www.mdpi.com/2223-7747/3/1/18