%0 Journal Article
%T Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures
%A St¨¦phanie Tissot
%A Patrik O. Michel
%A Clara J. Douet
%A Sarah Grezet
%A Lucia Baldi
%A David L. Hacker
%A Florian M. Wurm
%J Processes
%D 2013
%I MDPI AG
%R 10.3390/pr1010003
%X We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coef£¿cient of oxygen ( kLa) was 200%¨C400% higher in the HT tube than in a shake flask. Bacterial growth and plasmid production were 30% higher in the HT tube than in a 1-L Erlenmeyer flask. Mammalian cell cultures achieved a 25% higher cell density in the HT tube as compared to a 250-mL square-shaped bottle while insect cells grew as well in HT tubes as in 250-mL shake flasks. Because of their performance, disposability, and compact size, we conclude that 250-mL HT tubes are a useful alternative to other shaken containers for the cultivation of bacterial, mammalian and insect cells.
%K shake flasks
%K orbital shaking
%K bacteria
%K gas transfer
%K insect cells
%K mammalian cells
%U http://www.mdpi.com/2227-9717/1/1/3