%0 Journal Article
%T Development of a Freeze-Dried Skin Care Product Composed of Hyaluronic Acid and Poly(¦Ã-Glutamic Acid) Containing Bioactive Components for Application after Chemical Peels
%A Yuka Isago
%A Ryusuke Suzuki
%A Eri Isono
%A Yuya Noguchi
%A Yoshimitsu Kuroyanagi
%J Open Journal of Regenerative Medicine
%P 45-53
%@ 2169-2521
%D 2014
%I Scientific Research Publishing
%R 10.4236/ojrm.2014.33006
%X Eight types of spongy sheet were prepared by freeze-drying aqueous solutions of hyaluronic acid (HA) and poly(¦Ã-glutamic acid) (PGA) with or without bioactive components including vitamin C derivative (VC), glucosylceramide (GC), and epidermal growth factor (EGF). Spongy sheets were categorized into the following groups: Group I (HA/PGA), Group II (HA/PGA + VC), Group III (HA/PGA + GC), Group IV (HA/PGA + VC, GC), Group V (HA/PGA + EGF), Group VI (HA/PGA + VC, EGF), Group VII (HA/PGA + GC, EGF), and Group VIII (HA/PGA + VC, GC, EGF). In the first experiment, we examined fibroblast proliferation in conditioned medium that had been prepared by immersing each spongy sheet in a conventional culture medium. EGF-incorporating spongy sheets (Groups V-VIII) enhanced fibroblast proliferation more than EGF-free spongy sheets (Groups I-IV). In the second experiment, cytokine production by fibroblasts was evaluated using a wound surface model. This involved elevation of fibroblasts-incorporating collagen gel sheets to the air-liquid interface, on which a spongy sheet (Groups I, IV, V and VIII) was placed and cultured for 1 week. EGF-incorporating spongy sheets (Groups V and VIII) enhanced the production of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) by fibroblasts more than EGF-free spongy sheets (Groups I and IV). The effect of these four types of spongy sheet on wounds was investigated in animal experiments. Chemical peel was performed by contacting 50% trichloroacetic acid (TCA) on the dorsal region of mice, after which a spongy sheet was placed, and the wound condition was then observed in a two-week period. Angiogenesis was facilitated to a greater degree in Group VIII compared with Groups I, IV and V. This finding indicates that Group VIII spongy sheet is a promising aid for skin recovery after chemical peel.
%K Chemical Peel
%K Skin Care Product
%K Hyaluronic Acid
%K Poly(¦Ã-Glutamic Acid)
%K Vitamin C Derivative
%K Glucosylceramide
%K Epidermal Growth Factor
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=49441