%0 Journal Article
%T Ontogenic, Phenotypic, and Functional Characterization of XCR1+ Dendritic Cells Leads to a Consistent Classification of Intestinal Dendritic Cells Based on the Expression of XCR1 and SIRP¦Á
%A Steffen G¨¹ttler
%A Annabell Bachem
%A Evelyn Hartung
%A Ahmed Mora
%A Volker Henn
%A Richard A. Kroczek
%J Frontiers in Immunology
%D 2014
%I Frontiers Media
%R 10.3389/fimmu.2014.00326
%X In the past, lack of lineage markers confounded the classification of dendritic cells (DC) in the intestine and impeded a full understanding of their location and function. We have recently shown that the chemokine receptor XCR1 is a lineage marker for cross-presenting DC in the spleen. Now, we provide evidence that intestinal XCR1+ DC largely, but not fully, overlap with CD103+ CD11b£¿ DC, the hypothesized correlate of ¡°cross-presenting DC¡± in the intestine, and are selectively dependent in their development on the transcription factor Batf3. XCR1+ DC are located in the villi of the lamina propria of the small intestine, the T cell zones of Peyer¡¯s patches, and in the T cell zones and sinuses of the draining mesenteric lymph node. Functionally, we could demonstrate for the first time that XCR1+/CD103+ CD11b£¿ DC excel in the cross-presentation of orally applied antigen. Together, our data show that XCR1 is a lineage marker for cross-presenting DC also in the intestinal immune system. Further, extensive phenotypic analyses reveal that expression of the integrin SIRP¦Á consistently demarcates the XCR1£¿ DC population. We propose a simplified and consistent classification system for intestinal DC based on the expression of XCR1 and SIRP¦Á.
%K dendritic cells
%K XCR1
%K Batf3
%K SIRP¦Á
%K cross-presentation
%U http://www.frontiersin.org/Journal/10.3389/fimmu.2014.00326/abstract