%0 Journal Article
%T Aminated ¦Â-Cyclodextrin-Modified-Carboxylated Magnetic Cobalt/Nanocellulose Composite for Tumor-Targeted Gene Delivery
%A Thayyath Sreenivasan Anirudhan
%A Sylaja Raveendran Rejeena
%J Journal of Applied Chemistry
%D 2014
%R 10.1155/2014/184153
%X Gene therapy is a new kind of medicine, which uses genes as drugs in order to treat life threatening diseases. In the present work, a nonviral vector, aminated ¦Â-cyclodextrin-modified-carboxylated magnetic cobalt/nanocellulose composite (ACDC-Co/NCC), was synthesized for efficient transfection of genes into tumour cells. The synthesized ACDC-Co/NCC was characterized by means of FTIR, XRD, SEM, and ESR techniques. DNA condensing ability of ACDC-Co/NCC was found to be increased with increase in amount of ACDC-Co/NCC and 84.9% of DNA (1.0£¿¦Ìg/mL) inclusion was observed with 6.0£¿¦Ìg/mL of ACDC-Co/NCC. The cytotoxicity of ACDC-Co/NCC was observed to be minimal, even at higher concentration, with respect to the model transfecting agent, poly(ethyleneimine) (PEI). 88.2% of the gene was transfected at high dose of DNA, as indicated by the highest luciferase expression. These results indicated that ACDC-Co/NCC might be a promising candidate for gene delivery with the characteristics of good biocompatibility, potential biodegradability, minimal cytotoxicity, and relatively high gene transfection efficiency. 1. Introduction Gene delivery, the incorporation of foreign DNA particles into the host cells, is considered as one of the major objectives in the development of nucleic acid therapeutics. Gene therapy is a suitable substitute for conventional protein therapy because the problems such as bioavailability, systemic toxicity, manufacturing cost, and in vivo clearance rate can be solved [1]. It offers the opportunities and potential to cure both genetic and acquired diseases, such as hemophilia, cystic fibrosis, and cancers [2]. Previous experiments have confirmed that, at the molecular level, cancer is due to defects in the cellular DNA. The molecular pathogenesis of human cancer is due to structural and/or functional alterations of specific genes whose normal function is to control cell birth and cell death [3]. Gene delivery can be mediated by viral and nonviral methods. In viral methods, viruses deliver genetic materials to the specific disease sites via their efficient inherent mechanism. Viral vectors can provide efficient transduction and high gene expression. However, the use of viral vectors is quite limited due to safety concerns such as production of immunogenic reactions or mutagenesis of transfected cells [4]. These have necessitated the design of nonviral vectors as potential alternative which produces less complexity. Nonviral vectors are attractive gene delivery systems for tumour gene therapy [5]. These have low immunogenicity, are easier to
%U http://www.hindawi.com/journals/jac/2014/184153/