%0 Journal Article
%T Subacute Microcystin-LR Exposure Alters the Metabolism of Thyroid Hormones in Juvenile Zebrafish (Danio Rerio)
%A Zidong Liu
%A Rong Tang
%A Dapeng Li
%A Qing Hu
%A Ying Wang
%J Toxins
%P 337-352
%D 2015
%I MDPI AG
%R 10.3390/toxins7020337
%X Microcystin-LR (MC-LR) has been detected extensively in the aquatic environment and has the potential to disturb the thyroid endocrine system. However, limited information is available on the effects of subacute MC-LR exposure on fish thyroid hormone (TH) metabolism. In the present study, juvenile zebrafish ( Danio rerio) were exposed to MC-LR at environmentally relevant concentrations (0, 1, 5, and 25 ¦Ìg/L) for 28 days. Whole-body TH content and thyroid follicle histology were used as direct endpoints to assess thyroid disruption. The activities of iodothyronine deiodinases (IDs) and the transcription of selected genes associated with TH synthesis were also investigated to study the underlying mechanisms of endocrine disruption. Exposure of zebrafish to MC-LR significantly increased whole-body thyroxine (T 4) content but decreased whole-body triiodothyronine (T 3) content. We also observed hypertrophy and hyperplasia of the thyroid follicle epithelial cells, as well as up-regulation of corticotropin-releasing hormone (CRH), thyroid-stimulating hormone (TSH), thyroid peroxidase (TPO), and transthyretin (TTR) genes. The decreases in ID1 and ID2 activities coupled with an increase in ID3 activity were observed in MC-LR treatment groups. These results demonstrate that exposure to MC-LR at environmental concentrations results in the disturbance of TH homeostasis by disrupting the synthesis and conversion of THs.
%K microcystin-LR
%K thyroid hormones
%K histology
%K gene expression
%K iodothyronine deiodinases
%K zebrafish
%U http://www.mdpi.com/2072-6651/7/2/337