%0 Journal Article
%T Selective Recognition of 5-Hydroxytryptamine and Dopamine on a Multi-Walled Carbon Nanotube-Chitosan Hybrid Film-Modied Microelectrode Array
%A Huiren Xu
%A Li Wang
%A Jinping Luo
%A Yilin Song
%A Juntao Liu
%A Song Zhang
%A Xinxia Cai
%J Sensors
%P 1008-1021
%D 2015
%I MDPI AG
%R 10.3390/s150101008
%X It is dif£¿cult to determine dopamine (DA) and 5-hydroxytryptamine (5-HT) accurately because of the interference of ascorbic acid (AA) in vitro, which has a high concentration and can be oxidized at a potential close to DA and 5-HT at a conventional electrode, combined with the overlapping voltammetric signal of DA and 5-HT at a bare electrode. Herein, chitosan (CS) was used as a stabilizing matrix by electrochemical reaction, and multi-walled carbon nanotubes (MWCNTs) were modified onto the microelectrode array (MEA). The CS-MWCNT hybrid film-modified MEA was quite effective at simultaneously recognizing these species in a mixture and resolved the overlapping anodic peaks of AA, DA and 5-HT into three well-de£¿ned oxidation peaks in differential pulse voltammetry (DPV) at £¿80 mV, 105 mV and 300 mV ( versus Ag|AgCl), respectively. The linear responses were obtained in the range of 5 ¡Á 10 £¿6 M to 2 ¡Á 10 £¿4 M for DA (r = 0.996) and in the range of 1 ¡Á 10 £¿ 5 M to 3 ¡Á 10 £¿4 M for 5-HT (r = 0.999) using the DPV under the presence of a single substance. While DA coexisted with 5-HT in the interference of 3 ¡Á 10 £¿ 4 M AA, the linear responses were obtained in the range of 1 ¡Á 10 £¿5 M to 3 ¡Á 10 £¿4 M for selective molecular recognition of DA (r = 0.997) and 5-HT (r = 0.997) using the DPV. Therefore, this proposed MEA was successfully used for selective molecular recognition and determination of DA and 5-HT using the DPV, which has a potential application for real-time determination in vitro experiments.
%K microelectrode array
%K chitosan
%K multi-walled nanotubes
%K dopamine
%K 5-hydroxytryptamine
%U http://www.mdpi.com/1424-8220/15/1/1008