%0 Journal Article
%T Fluorescent Cellular Assay for Screening Agents Inhibiting Pseudomonas aeruginosa Adherence
%A Libue Noskov¨˘
%A Boena Kub¨Şkov¨˘
%A Lucie Vakov¨˘
%A Barbora Bl¨˘hov¨˘
%A Michaela Wimmerov¨˘
%A Marie Stiborov¨˘
%A Petr Hodek
%J Sensors
%P 1945-1953
%D 2015
%I MDPI AG
%R 10.3390/s150101945
%X Antibodies against Pseudomonas aeruginosa (PA) lectin, PAIIL, which is a virulence factor mediating the bacteria binding to epithelium cells, were prepared in chickens and purified from egg yolks. To examine these antibodies as a prophylactic agent preventing the adhesion of PA we developed a well plate assay based on fluorescently labeled bacteria and immortalized epithelium cell lines derived from normal and cystic fibrosis (CF) human lungs. The antibodies significantly inhibited bacteria adhesion (up to 50%) in both cell lines. In agreement with in vivo data, our plate assay showed higher susceptibility of CF cells towards the PA adhesion as compared to normal epithelium. This finding proved the reliability of the developed experimental system.
%K cystic fibrosis
%K lungs
%K bacterial infection
%K lectin
%K chicken antibody
%K cell line
%K dual fluorescence
%U http://www.mdpi.com/1424-8220/15/1/1945