%0 Journal Article
%T Isolation and Characterization of Plant Growth Promoting Rhizobacteria (PGPR) from Rice Rhizosphere of Varanasi and East Champaran District of India
%A Umesh Prasad Shrivastava
%J Academic Voices: A Multidisciplinary Journal
%P 81-90
%D 2013
%R 10.3126/av.v3i1.9542
%X Keeping with objective to search efficient PGPR isolates, 46 bacterial isolates were isolated from the rhizosphere of 17 rice fields of two districts of India employing standard enrichment techniques using£¿ JNFb- solid agar medium. Among all isolates, ECI-10A showed highest nitrogenase activity (1.72 ¡À 0.09 mmol C2H4 formed/mg protein/h) and ECI-2A showed lowest activity (0.62 ¡À 0.08 mmol C2H4 formed/mg protein/h). IAA production, P-solubilization and siderophore production were also tested among these isolates. Highest £¿IAA production, P-solubilization and siderophore production were recorded in AF-7C (7.36 ¡À 0.38 ¦Ìg/mg dry weight), ECI-12A (188.65 ¡À 8.65 ¦Ìg/mg dry weight) and ECI-10B (20.43 ¡À 3.94 ¦Ìg/ mg dry weight) respectively; whereas, lowest in AF-4B (2.3 ¡À 0.3 ¦Ìg/mg dry weight), £¿AF-1C (12.47 ¡À 3.5¦Ìg/mg dry weight) and AF-4B (1.45 ¡À 2.32 ¦Ìg/ mg dry weight) respectively. Pseudomonas, Klebsiella, Azotobacter and Agrobacterium were predominantly present in the rhizosphere of rice plants of both districts of India is reported in present investigation.
%K Plant growth promoting rhizobacteria
%K PGPR
%K Rhizosphere
%K Beneficial rhizobacteria
%K Acetylene reduction assay
%U http://www.nepjol.info/nepal/index.php/AV/article/view/9542