%0 Journal Article
%T Cytotoxicity, Total Phenolic Content and Antioxidant Activity of Bergenia purpurascens Rhizome
%A Gan B Bajracharya
%A Rokesh Maharjan
%J Nepal Journal of Science and Technology
%P 87-94
%D 2013
%R 10.3126/njst.v14i1.8927
%X This study was aimed to evaluate the cytotoxicity of methanolic extract (ME) and aqueous extract (AE) of the rhizomes of Bergenia purpurascens through the Brine-shrimp bioassay, which revealed that ME possessed cytotoxicity with LC 50 = 181.97 ìg/ml value. The plant extracts were also evaluated in vitro for antioxidant activity using different redox [Folin-Ciocalteu (FC) and Ferric Reducing Antioxidant Power (FRAP)] and radical scavenging [(2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)] assays. Both ME and AE possessed antioxidant activity and ME displayed comparatively a greater potential. The total phenolic contents in ME and AE was 1.27 and 0.06 g gallic acid equivalent (GAE)/100 g dry mass (DM), respectively. The FRAP values determined for ME and AE were 15.82 and 2.58 ìmol Fe(II)/100 g DM, respectively. The DPPH radical scavenging effect (IC 50 ) was for ME 19.86 ìg/ml and 295.91 ìg/ml for AE. At 100 ìg/ml concentration, up to 94% inhibition of ABTS + was observed in 6 min incubation. Nepal Journal of Science and Technology Vol. 14, No. 1 (2013) 87-94 DOI: http://dx.doi.org/10.3126/njst.v14i1.8927
%K antioxidant activity
%K Bergenia purpurascens
%K brine-shrimp bioassay
%K free radical
%K phenolic content
%U http://www.nepjol.info/index.php/NJST/article/view/8927