%0 Journal Article
%T Automated Measurement of Immature Granulocytes: Performance Characteristics and Utility in Routine Clinical Practice
%A Balamurugan Senthilnayagam
%A Treshul Kumar
%A Jayapriya Sukumaran
%A Jeya M.
%A Ramesh Rao K.
%J Pathology Research International
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/483670
%X The granulocytic ¡°shift to left¡± reflects marrow response to bacterial infection, and this may be quantified as band count or immature granulocyte count (IGC). The former value, used widely in neonatal sepsis, has been notoriously difficult to measure accurately and precisely. A reproducible, precise, and accurate counting of immature granulocyte counts may be possible with automation. This study of 200 febrile patients aimed at analysing the performance characteristics of automated immature granulocytes (AIGs) in predicting blood culture and their clinical utility. The absolute (IGC) and relative IG count (IG%) had area under curve (AUC) of 0.69 and 0.66. Moreover, the means of IGC and IG% between culture positive and negative groups were statistically significant suggesting that they are potential markers for bacteremia. IGC of 0.03£¿¡Á£¿103£¿cu¡¤mm and IG% of 0.5% offered sensitivity of 86.3% and 92.2%, respectively, and may be used for screening for bacteremia. Higher values, IGC£¿>£¿0.3, and IG%£¿>£¿3 had specificity greater than 90%, although the values were infrequent. It may not be long before that these automated hemograms are put into regular diagnostic use. 1. Introduction Early detection of bacteremia facilitates timely initiation of antimicrobial therapy, reduces morbidity and mortality, and decreases healthcare costs thereby making it a relevant clinical objective. However, there is a considerable timelag before the blood culture results are available for the physician to act upon. This has necessitated studies to address the usefulness of various parameters to predict infection earlier [1]. The manual ¡°band count¡± used widely in pediatric practice as a marker for bacterial infection has been notoriously difficult to measure accurately and precisely [2, 3]. Therefore, a more reproducible measurement of immature granulocytes might be a useful parameter to predict infection or sepsis. Automated hematology analysers have undergone numerous technical innovations during the last few years. Recent developments permit not only flagging of samples with abnormal cell population but also categorisation and counting of those cells [4]. The Coulter Act Diff 5 counter can perform a 5-part differential leucocyte count and can also enumerate the percentage and absolute number of immature granulocytes (IG% and IGC) using a technology that combines cytochemistry, focused flow impedance, and light absorbance [5, 6]. At this time, automated immature granulocyte measurements are still being evaluated in the research arena and do not form part of routine reporting.
%U http://www.hindawi.com/journals/pri/2012/483670/