%0 Journal Article
%T Hydrophilic/hydrophobiccharactersofantimicrobialpeptidesderivedfromanimalsandtheireffectsonmultidrugresistantclinicalisolates
%A Cun-Bao LIU
%A Bin SHAN
%A Hong-Mei BAI
%A Jing TANG
%A Long-Zong YAN
%A Yan-Bing MA
%J 动物学研究
%P 41-47
%D 2015
%R 10.13918/j.issn.2095-8137.2015.1.41
%X Multidrugresistant(MDR)pathogeninfectionsareseriousthreatstohospitalizedpatientsbecauseofthelimitedtherapeuticoptions.Anovelgroupofantibioticcandidates,antimicrobialpeptides(AMPs),haverecentlyshownpowerfulactivitiesagainstbothGram-negativeandGram-positivebacteria.Unfortunately,theviabilityofusingtheseAMPsinclinicalsettingsremainstobeseen,sincemoststillneedtobeevaluatedpriortoclinicaltrialsandnotallofAMPsarepotentagainstMDRclinicalisolates.TofindaconnectionbetweenthecharacteristicsofseveraloftheseAMPsandtheireffectsagainstMDRpathogens,weselected14AMPsofanimaloriginwithtypicalstructuresandevaluatedtheirinvitroactivitiesagainstclinicalstrainsofextensivedrug-resistantAcinetobacterbaumannii,methicillin-resistantStaphylococcusaureus,extendedspectrumβ-lactamase-producingPseudomonasaeruginosaandextendedspectrumβ-lactamase-producingEscherichiacoli.Ourresultsshowedthatthesepeptides'hydrophilic/hydrophobiccharacteristics,ratherthantheirsecondarystructures,mayexplaintheirantibacterialeffectsontheseclinicalisolates.PeptidesthatareamphipathicalongthelongitudinaldirectionseemedtobeeffectiveagainstGram-negativepathogens,whilepeptideswithhydrophilicterminalsseparatedbyahydrophobicintermediatesectionappearedtobeeffectiveagainstbothGram-negativeandGram-positivepathogens.Amongthese,cathelicidin-BFwasfoundtoinhibitalloftheGram-negativepathogenstestedatdosagesofnomorethan16mg/L,killingapandrug-resistantA.baumanniistrainwithin2hat4×MICsand4hat2×MICs.TachyplesinIIIwasalsofoundcapableofinhibitingallGram-negativeandGram-positivepathogenstestedatnomorethan16mg/L,andsimilarlykilledthesameA.baumanniistrainwithin4hat4×MICsand2×MICs.Theseresultssuggestthatbothcathelicidin-BFandtachyplesinIIIarelikelyviabletargetsforthedevelopmentofAMPsforclinicaluses.
%K Hydrophilic/hydrophobiccharacter
%K Multidrugresistantclinicalisolate
%K Cathelicidin-BF
%K TachyplesinIII
%U http://www.zoores.ac.cn/CN/abstract/abstract3561.shtml