%0 Journal Article
%T MolecularcharacterizationofanIL-1βgenefromthelargeyellowcroaker(Larimichthyscrocea)anditseffectonfishdefenseagainstVibrioalginolyticusinfection
%A Jun WU
%A Yu-Hong SHI
%A Xue-Heng ZHANG
%A Chang-Hong LI
%A Ming-Yun LI
%A Jiong CHEN
%J 动物学研究
%P 133-141
%D 2015
%X Interleukin1β(IL-1β),thefirstinterleukintobecharacterized,playsakeyroleinregulatingtheimmuneresponse.Inthisstudy,wedeterminedthecDNAandgenomicDNAsequencesoftheIL-1βgenefromthelargeyellowcroaker,Larimichthyscrocea.PhylogeneticanalysisindicatedthattheIL-1β(LcIL-1β)genewasmostcloselyrelatedtothatofEuropeanseabass(Dicentrarchuslabrax),sharing67.8%aminoacididentity.Inhealthylargeyellowcroaker,LcIL-1βtranscriptionwasdetectedinalltestedtissues,withthehighestlevelfoundintheheadkidney.UponVibrioalginolyticusinfection,LcIL-1βtranscriptioninalltestedtissueswassignificantlyupregulated.IntraperitonealinjectionofrecombinantLcIL-1β(rLcIL-1β)improvedthesurvivalrateandreducedthetissuebacterialloadafterV.alginolyticusinfection.Inaddition,rLcIL-1βinducedmonocytes/macrophages(MO/MΦ)chemotaxisandincreasedphagocytosisandbactericidalactivityinvitro.TheseresultssuggestthatLcIL-1βplaysanimportantroleinthelargeyellowcroakerimmuneresponseagainstV.alginolyticus.
%K Interleukin1&beta
%K Largeyellowcroaker
%K Survivalrate
%K Vibrioalginolyticus
%K Monocytes/macrophages
%U http://www.zoores.ac.cn/CN/abstract/abstract3639.shtml