%0 Journal Article
%T A New Method for     D   2   O    /    H   2   O     Exchange in Infrared Spectroscopy of Proteins
%A Marco Malferrari
%A Giovanni Venturoli
%A Francesco Francia
%A Alberto Mezzetti
%J Spectroscopy: An International Journal
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/791342
%X In this paper, we describe a new method to obtain D2O/H2O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides. The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2O needed; (iii) the short time required for dehydration and D2O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample. The proposed method can be applied to other proteins.
%U http://www.hindawi.com/journals/spectroscopy/2012/791342/