%0 Journal Article
%T Determination of Nanogram Quantities of Emodin in Pharmaceutical Preparations and Biofluids by Luminol-Myoglobin Chemiluminescence System
%A Yun Zhang
%A Xili He
%A Zhenghua Song
%J Spectroscopy: An International Journal
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/741053
%X Based on the quenching effect of emodin on the luminol-myoglobin (Mb) reaction, a sensitive method for the determination of nanogram level emodin by flow injection chemiluminescence (FI-CL) is presented for the first time. It was found that the CL intensity from luminol-Mb system could be inhibited in the presence of emodin, and the decrement of CL intensity was linearly proportional to the logarithm of emodin concentration in the range of 0.5–300ng£¿mL£¿1 (R = 0.9956) with the detection limit of 0.2ng£¿mL£¿1 (3σ). At a flow rate of 2.0mL£¿min£¿1, a complete determination of emodin, including sampling and washing, could be accomplished in 0.5 min with the relative standard deviations (RSDs) of less than 3.5% (n = 5). The proposed method was successfully applied to the determination of emodin in pharmaceutical preparations and human serum samples. The possible CL mechanism of luminol-Mb-emodin reaction was explained.
%U http://www.hindawi.com/journals/spectroscopy/2012/741053/