%0 Journal Article
%T Antimicrobial Peptide from the Eusocial Bee Halictus sexcinctus Interacting with Model Membranes
%A Mark¨¦ta Pazderkov¨¢
%A Eva Ko i ov¨¢
%A Tom¨¢  Pazderka
%A Petr Malo¨¾
%A Vladim¨ªr Kopecky Jr.
%A Lenka Monincov¨¢
%A V¨¢clav  e ovsky
%A Lucie Bedn¨¢rov¨¢
%J Spectroscopy: An International Journal
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/840956
%X Halictine-1 (Hal-1)—a linear antibacterial dodecapeptide isolated from the venom of the eusocial bee Halictus sexcinctus—has been subjected to a detailed spectroscopic study including circular dichroism, fluorescence, and vibrational spectroscopy. We investigated Hal-1 ability to adopt an amphipathic α-helical structure upon interaction with model lipid-based bacterial membranes (phosphatidylcholine/phosphatidylglycerol-based large unilamellar vesicles and sodium dodecylsulfate micelles) and helix inducing components (trifluoroethanol). It was found that Hal-1 responds sensitively to the composition of the membrane model and to the peptide/lipid ratio. The amphipathic nature of the helical Hal-1 seems to favour flat charged surfaces of the model lipid particles over the nondirectional interaction with trifluoroethanol. Increasing fraction of polyproline II type conformation was detected at low peptide/lipid ratios.
%U http://www.hindawi.com/journals/spectroscopy/2012/840956/