%0 Journal Article
%T Validated UPLC/Q-TOF-MS Method for Determination of Poliumoside in Rat Plasma and Its Application to Pharmacokinetic Study
%A Hao Qian
%A Danyi Lu
%A Wan Li
%A Xiaotong Zhou
%A Baojian Wu
%A Zhiguo Ma
%J American Journal of Analytical Chemistry
%P 266-274
%@ 2156-8278
%D 2016
%I Scientific Research Publishing
%R 10.4236/ajac.2016.73024
%X Poliumoside is the
main active constituent of <i>Callicarpa
kwangtungensis</i> Chun (CK), a traditional Chinese medicine for management of
hemostasis. In this study, a rapid and selective ultra-performance liquid chromatography coupled with
quadrupole time-of-flight mass spectrometry (UPLC/ Q-TOF-MS) method was
developed and validated to quantify poliumoside in rat plasma. The targeted
analytes in rat plasma were prepared through protein-precipitation method using
10% trichloroacetic acid (TCA). The chromatographic separation was performed on a Waters BEH C<sub>18</sub> column (2.1 ¡Á 100 mm, 1.7 ¦Ìm) by acetonitrile-water containing 0.1% formic
acid. The calibration curve was linear over the range of 50 - 10,000 ng/mL (r<sup>2</sup> &gt; 0.99). The intra-day or inter-day precision was less than 7.97% and
accuracy was within ?7.00% - 3.36%. The developed method was successfully
applied to pharmacokinetic study of poliumoside in rat plasma. Although being
rapidly absorbed (T<sub>max</sub> ¡Ü 30 min), poliumoside was poorly bioavailable
after oral administration (the absolute bioavailability was only 0.69%).
%K Poliumoside
%K UPLC/Q-TOF-MS
%K Rat Plasma
%K Pharmacokinetics
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=64530