%0 Journal Article
%T Bioactive Compounds Produced by <i>Streptomyces</i> sp. Isolate UPMRS4 and Antifungal Activity against <i>Pyricularia oryzae</i>
%A Hayman Kakakhan Awla
%A Jugah Kadir
%A Radziah Othman
%A Tavga Sulaiman Rashid
%A Mui-Yun Wong
%J American Journal of Plant Sciences
%P 1077-1085
%@ 2158-2750
%D 2016
%I Scientific Research Publishing
%R 10.4236/ajps.2016.77103
%X A </span><i><span style=\"font-family:Verdana;\">Streptomyces </span></i><span style=\"font-family:Verdana;\">isolate having
antifungal activity against </span><i><span style=\"font-family:Verdana;\">Pyricularia oryzae</span></i><span style=\"font-family:Verdana;\">, the causal agent of rice blast disease, was isolated from </span></span><span style=\"font-family:Verdana;\">soil
collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia.</span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">The aim of the study was
to determine the antifungal activity of </span><i><span style=\"font-family:Verdana;\">Streptomyces</span></i><span style=\"font-family:Verdana;\"> sp. isolate UPMRS4
extracts against </span><i><span style=\"font-family:Verdana;\">P. oryzae</span></i><span style=\"font-family:Verdana;\"> and to identify bioactive antifungal
compounds produced by UPMRS4. Various solvents were used for extraction of
antifungal compounds and well diffusion method was used to determine the
antifungal activity of the extracts. The ethyl acetate extract demonstrated the
highest activity against mycelial growth of </span><i><span style=\"font-family:Verdana;\">P.
oryzae</span></i><span style=\"font-family:Verdana;\">, with an effective inhibitory concentration (EIC) of 1.562 ¦Ìg/ml
significantly higher compared to that of chloroform, diethyl ether, methanol,
acetone, ethanol and water. Based on GC-MS and LC-MS/MSanalyses,</span></span><span style=\"font-family:Verdana;\">compounds
with antifungal activity </span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">were detected such as (Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-</span><span style=\"font-family:Verdana;\">3-(2-methylpropyl); Pyrrolo[1,2-a]
pyrazine-1,4-dione, hexahydro-3-(phenylmethyl); ergotamine; </span></span><span style=\"font-family:Verdana;\">amicomacin</span><span style=\"font-family:Verdana;\">; </span><span style=\"font-family:Verdana;\">fungichromin;
rapamycin</span><span style=\"font-family:Verdana;\"> and</span><span style=\"font-family:Verdana;\"> N-Acetyl-D,
L-phenylalanine</span><span style=\"font-family:Verdana;\">. </span><span style=\"font-family:Verdana;\">These compounds had good
general antifungal activity and might have potential future agricultural applications.
%K GCMS
%K LCMS/MS
%K Antifungal
%K &lt
%K i&gt
%K Streptomyces&lt
%K /i&gt
%K sp. and Rice Blast
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=66540