%0 Journal Article
%T Production and Characterization of Recombinant Rat Non-Collagen  Domain of <i>¦Á</i>3 Chain of Type IV  Collagen <i>¦Á</i>3 (IV) NC1 Antigen
%A Afsana Munni
%J CellBio
%P 27-48
%@ 2325-7792
%D 2016
%I Scientific Research Publishing
%R 10.4236/cellbio.2016.53003
%X The glomerulonephritis disease is characterized by inflammation of glomeruli or small blood vessels in the kidney that causes kidney diseases. The reason of glomerulonephritis disease is to deposit the anti-GBM auto antibody in the glomerular basement membrane. The type IV collagen is the main component of glomerular basement membrane that has <em><span class=\"KSFIND_CLASS\" id=\"1KSFindDIV\">¦Á</span></em>3 chain of type (IV) collagen of non-collagenous domain which contains N-terminal 7S domain, a triple helical collagenous domain and C-terminal non-collagenous glomerular domain (NC1). The amino terminal of <em><span class=\"KSFIND_CLASS\" id=\"2KSFindDIV\">¦Á</span></em>3 (IV) NC1 that induces the Experimental Autoimmuno Glomerulonephritis (EAG) in rat model has been identified. The recombinant rat <span class=\"KSFIND_CLASS_SELECT\" id=\"0KSFindDIV\"><em>¦Á</em></span>3 (IV) NC1 antigen has nine amino acid spans that are consistent with antibody or T cell epitope that induces in EAG. The research is carried out on the recombinant rat <em><span class=\"KSFIND_CLASS\" id=\"3KSFindDIV\">¦Á</span></em>3 (IV) NC1 production, purification, quantification, and characterization. The circulation of anti-GBM antibody in glomerular basement membrane can be measured by the ELISA assay. In addition, the recombinant rat antigen is secreted in HEK293 cell supernatant that is purified by Anti-FLAG M2 monoclonal IgG antibody affinity column and characterized and quantified by SDS-PAGE gel electrophoresis and Western blotting techniques.
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%K Auto-Immuno Kidney Disease
%K Glomerulonephritis Disease
%K Glomerular Basement Membrane
%K &lt
%K i&gt
%K ¦Á&lt
%K /i&gt
%K 3 (IV) NC1-Non-Collagen Domain of &lt
%K i&gt
%K ¦Á&lt
%K /i&gt
%K 3 Chain of Type IV Collagen &lt
%K i&gt
%K ¦Á&lt
%K /i&gt
%K 3 (IV)
%K Antibody(Ab)
%K Antigen (Ag)
%K Anti Glomerular Basement Membrane
%K Experimental Autoimmune Glomerulonephritis
%K Enzyme-Linked Immunosorbent Assay (ELISA)
%K Human Embryonic Kidney (HEK)
%K Ig-Immunoglobulin (IgG
%K IgA)
%K IgAN-IgA nephropathy
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=69960