%0 Journal Article
%T Effect of Alteration of Glutathione Content on Cell Viability in ¦Á-Synuclein-Transfected SH-SY5Y Cells
%A Ken-Ichi Tanaka
%A Kanako Sonoda
%A Masato Asanuma
%J Advances in Parkinson's Disease
%P 93-100
%@ 2169-9720
%D 2017
%I Scientific Research Publishing
%R 10.4236/apd.2017.63010
%X It is well known that ¦Á-synuclein (¦ÁS) plays an important role in the pathogenesis
of Parkinson¡¯s disease (PD). Moreover, oxidative stress is also thought to
be an important factor in PD due to induction of dopaminergic neuronal cell
death by free radicals and enhancement of ¦ÁS fibrillation by oxidized stress. In
the present study, to clarify the role of glutathione (GSH), an intracellular antioxidant,
on the molecular mechanism of ¦ÁS-induced cell injury, we examined
the effects of L-buthionine-SR-sulfoximine (BSO), a GSH synthase
inhibitor, with or without N-acetyl-L-cysteine (NAC), a source of GSH, on
¦ÁS-induced cell injury in human neuroblastoma SH-SY5Y cells. Treatment with
BSO significantly reduced the cell viability of both empty-vector- and ¦ÁS-transfected
SH-SY5Y cells in a dose-dependent manner (p < 0.01), although the ratio
of ¦ÁS-induced reduction of cell viability in ¦Á-syn-transfected cells was much
greater than that in empty-vector-transfected cells. Moreover, BSO significantly
reduced the intracellular total GSH level in both types of transformant cells.
However, NAC significantly prevented BSO-induced reduction of both cell viability
and GSH level in the ¦ÁS-transfected cells. These findings suggest that GSH
plays an important role in ¦ÁS-induced cell injury by reducing cell viability.
%K ¦Á-Synuclein
%K L-Buthionine-SR-Sulfoximine
%K N-Acetyl-L-Cysteine
%K Glutathione
%K SH-SY5Y Cells
%K Parkinson¡¯s Disease
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=78635