%0 Journal Article
%T <i>In Vitro</i> Study of the Nephrotoxicity of Tripterygium Tablet Extract and Triptolide in Monolayer HK-2 Cells Cultured in a Transwell Chamber
%A Ran Hao
%A Lianqiang Hui
%A Chun Li
%A Chunyu Cao
%A Yifei Yang
%A Jiyuan Zhang
%A Ting Liu
%A Yi Zhang
%J Chinese Medicine
%P 34-54
%@ 2151-1926
%D 2018
%I Scientific Research Publishing
%R 10.4236/cm.2018.91003
%X We established a monolayer polarized cell model
using human kidney 2 (HK-2) cells cultured in a transwell chamber to examine
the changes in the morphology and physiological functions of human-derived
renal proximal tubular epithelial cells caused by tripterygium tablet extract
(TTE) and triptolide. HK-2 cells were cultured on PCF membranes to form a
complete monolayer of cells. A MTT assay was used to select 10, 40, 160, 640 μg，ml-1 TTE or 4, 16, 64, 256 ng，ml-1 triptolide to treat HK-2 monolayer cells. After 24 hours, a FITC permeability
assay was performed; GGT, LDH and NAG secretion on the apical (AP) and basolateral
(BL) sides of the cells by HK-2 cells were examined. The morphology and the
monolayer structure of HK-2 cells was observed via optical microscope and
scanning electron microscope, respectively. The effect on the cytoskeleton of
HK-2 cells was observed under a fluorescence microscope. The IC50 of
TTE was 277.122 μg，ml-1, and the IC50 of
triptolide was 148.035 ng，ml-1. Compared with the DMSO
group, the FITC leakage rate with TTE 160, 640 μg，ml-1 treated
group and 4 - 256 ng，ml-1 triptolide dose group exhibited
statistically significant increase. TTE significantly increased secretion of
GGT and LDH at 160, 640 μg，ml-1, meanwhile, dramatically
increased the AP/BL ratio of LDH at 160 μg，ml-1;
triptolide significantly increased secretion and AP/BL ratio of GGT and LDH at
256 ng，ml-1. The morphological observations via optical
and electron
%K Nephrotoxicity
%K Tripterygium Tablet Extract
%K Triptolide
%K HK-2 Cell
%K Transwell Chamber
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=83001