%0 Journal Article
%T One-Step Procedure for Direct Purification of Pediocin-Like Bacteriocins and Cationic Antimicrobial Peptides from Complex Culture Medium on an Analytical, Semipreparative, and Preparative Scale. A New Type of Liquid Chromatography¡ªColloidal High-Performance Liquid Chromatography (CHPLC)
%A Ilia Brondz
%J International Journal of Analytical Mass Spectrometry and Chromatography
%P 41-49
%@ 2332-1776
%D 2018
%I Scientific Research Publishing
%R 10.4236/ijamsc.2018.63004
%X A simple time- and labor-saving one-step procedure suitable for analytical-, semipreparative-, and preparative-scale preparation of bacteriocins and other cationic antibacterial peptides (for example as lysozyme) has been developed. Suspensions of bacterial cells, other colloidal- and fast-particle materials, and dissolved anionic molecules passed through the column; however, cationic bacteriocins were separated by chromatography. The purity of the target substance at more than 97% was achieved by the judgment of spectral, chromatographic, and antimicrobial activity. A many-fold concentration increase was achieved for bacteriocins, lysozyme, and bacitracin (a commercial antibiotic) in comparison with the starting solutions (see the text below). In the experiments, homemade cation-exchange columns have been used. The columns have been prepared by the grafting of glass fibers with a diameter of 0.2 ¦Ìm. In this paper, applications of a new type of liquid chromatography¡ª<em>colloidal high-performance liquid chromatography</em> (CHPLC)¡ªwere demonstrated.
%K Liquid Chromatography
%K Bacterial Cells
%K Bacteriocins
%K Antibacterial Peptides
%K Lysozyme
%K Bacitracin
%K Cation-Exchange Columns
%K Grafting
%K Glass Fibers
%K New Type of Liquid Chromatography
%K Colloidal High-Performance Liquid Chromatography
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=87148