%0 Journal Article
%T Influence of Agitation and Aeration on Single Cell Oil Production by <i>Rhodotorula glutinis</i> from Glycerol
%A Tatiane F. S. R. Bento
%A Vitor F. M. Viana
%A Livia M. Carneiro
%A Jo£¿o Paulo A. Silva
%J Journal of Sustainable Bioenergy Systems
%P 29-43
%@ 2165-4018
%D 2019
%I Scientific Research Publishing
%R 10.4236/jsbs.2019.92003
%X In the present study, the yeast <em>Rhodotorula glutinis</em> has been assessed with the aim of producing microbial lipids from glycerol under different aeration conditions. For such a purpose, experiments were carried out in shake flasks, under different conditions of agitation (150 to 250 rpm) and aeration (2.5 to 5.0 of flask volume-to-medium volume ratio). Furthermore, their influence on fermentative parameters (lipid and cell concentration, biomass yield; lipid yield; and lipid volumetric productivity) has been investigated using a 2<sup>2</sup> full factorial design. The statistical analysis has revealed a strong influence of both variables on substrate consumption, lipid accumulation, cell growth and lipid productivity. As a whole, results suggest that higher aeration levels provide greater cell and lipid concentrations, and lipid volumetric productivity. The best results (4.5 g/L of lipids and Q<sub>P </sub>= 0.95 g/L<span style=\"white-space:nowrap;\">&amp;sdot;</span>day) were achieved at the highest aeration (5.0 flask volume-to-medium volume ratio) and agitation (250 rpm) levels. Their fatty acid profile showed that oleic acid was produced in greater quantity (53.5%), followed by linoleic acid (18.7%), palmitic acid (6.8%) and stearic acid (9.9%). The microbial oil presented viscosity of 39.3 cP at 50¡ãC and free fatty acid content of 1.93% ¡À 0.08%. These are significant results and contribute to establishing operational conditions that maximize single-cell oil production from glycerol by <em>Rhodotorula glutinis,</em> <em>i.e.</em> an alternative source as renewable raw material for lipid-based biorefineries.
%K Single-Cell Oil
%K &lt
%K i&gt
%K Rhodotorula glutinis&lt
%K /i&gt
%K Aeration
%K Glycerol
%K Oleaginous Yeast
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=92310