%0 Journal Article
%T Effects of Mitochondrial DNA Deletion and OSW-1 on PI3K-AKT Signaling Pathway PCR Array in SK-Hep1 Cells
%A Xichen Du
%A Changshi Qian
%A Xinglin Jin
%J Chinese Medicine
%P 107-119
%@ 2151-1926
%D 2019
%I Scientific Research Publishing
%R 10.4236/cm.2019.103006
%X To investigate the effect of mitochondrial DNA
deletion and OSW-1 on PI3K-AKT signaling pathway PCR Array in SK-Hep1
hepatocellular carcinoma cells</span><span style=\"font-family:Verdana;\">,</span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> we prepared
SK-Hep1 cells with </span><span style=\"font-family:Verdana;\">mtDNA</span></span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:Verdana;\">deletion</span><span style=\"font-family:Verdana;\">, that is</span><span style=\"font-family:Verdana;\">,</span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> p</span><sup><span style=\"font-family:Verdana;\">0</span></sup><span style=\"font-family:Verdana;\">SK Hep. Then the OSW-1 of 100</span></span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">ng/L was used to intervene SK-Hep1 and p</span><sup><span style=\"font-family:Verdana;\">0</span></sup><span style=\"font-family:Verdana;\">SK-Hep1.
RT-qPCR was used to detect the difference of gene expression on</span></span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:Verdana;\">PI3K-AKT signaling pathway
PCR Array</span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:Verdana;\">in four
groups of cells. The gene expression of TLR4, FOS and TSC2 markers</span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:Verdana;\">in SK-Hep1 cells treated
with OSW-1 were significantly increased. The gene expression</span><span style=\"font-family:Verdana;\">s</span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> of PDPK1, GJA1, TLR4 </span><span style=\"font-family:Verdana;\">and</span><span style=\"font-family:Verdana;\"> TSC2 markers</span></span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:Verdana;\">w</span><span style=\"font-family:Verdana;\">ere</span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> significantly increased in p</span><sup><span style=\"font-family:Verdana;\">0</span></sup><span style=\"font-family:Verdana;\">SK-Hep1 cells, and the gene
expression</span></span><span style=\"font-family:Verdana;\">s</span><span style=\"font-family:Verdana;\"> of IRAK1
and GJA1 markers w</span><span style=\"font-family:Verdana;\">ere</span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> significantly increased in p</span><sup><span style=\"font-family:Verdana;\">0</span></sup><span style=\"font-family:Verdana;\">SK-Hep1 cells
%K mtDNA
%K OSW-1
%K PI3K-AKT
%K PCR Array
%K SK-Hep1 Cells
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=95403