%0 Journal Article
%T Evaluation of the TbgI<sub>2</sub> and TbgI<sub>17</sub> Tandem Repeat Antigens as Potential Antigens for the Diagnosis of <i>Trypanosoma brucei rhodesiense</i>
%A Vanessa Irumva
%A Rebecca Waihenya
%A Anne Wanjiru Mwangi
%A Peter Kipkemboi
%A Satoshi Kaneko
%A Tonny Teya
%A Joanne Jepkemei
%A Caroline Njoroge
%A Joseph Wambugu Gitari
%A Matthew Mutinda Munyao
%A Robinson M. Irekwa
%A M£¿r£¿ma Ng¡¯ang¡¯a
%A Samson Muuo Nzou
%J Open Journal of Clinical Diagnostics
%P 152-163
%@ 2162-5824
%D 2019
%I Scientific Research Publishing
%R 10.4236/ojcd.2019.94011
%X Human African trypanosomiasis (HAT) affects up to
half a million people every year in sub-Saharan Africa. Interruption of
transmission of the disease by early diagnosis and treatment is core to the
control and eventual elimination of HAT. The routine diagnostic method for HAT
is light microscopy of blood samples. The present study sought to evaluate the
potential of TbgI</span><sub><span style=\"font-family:Verdana;\">2</span></sub><span style=\"font-family:Verdana;\"> and TbgI</span><sub><span style=\"font-family:Verdana;\">17</span></sub><span style=\"font-family:Verdana;\"> tandem repeat antigens as
candidates for the diagnosis of </span><i><span style=\"font-family:Verdana;\">Trypanosoma
brucei</span></i> </span><i><span style=\"font-family:Verdana;\">r</span></i><i><span style=\"font-family:Verdana;\">hodesiense</span></i><span style=\"font-family:Verdana;\">. The expressed proteins were purified and the
antigenic reactivity evaluation </span><span style=\"font-family:Verdana;\">was </span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">done using multiplex assay using sera obtained from HAT patients.
Receiver operating characteristic analysis showed that recombinant antigen,
TbgI</span><sub><span style=\"font-family:Verdana;\">2</span></sub><span style=\"font-family:Verdana;\"> had high sensitivity for sera from patients infected with </span><i><span style=\"font-family:Verdana;\">T</span></i><span style=\"font-family:Verdana;\">. </span><i><span style=\"font-family:Verdana;\">b</span></i><span style=\"font-family:Verdana;\">. </span></span><i><span style=\"font-family:Verdana;\">r</span></i><i><span style=\"font-family:Verdana;\">hodesiense</span></i><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> with the
area under the curve being 0.577 and a sensitivity of 0.641 and specificity 0.650.
The results suggest that TbgI</span><sub><span style=\"font-family:Verdana;\">2</span></sub><span style=\"font-family:Verdana;\"> is a potential biomarker for </span><i><span style=\"font-family:Verdana;\">T</span></i><span style=\"font-family:Verdana;\">. </span><i><span style=\"font-family:Verdana;\">b</span></i><span style=\"font-family:Verdana;\">. </span></span><i><span style=\"font-family:Verdana;\">r</span></i><i><span style=\"font-family:Verdana;\">hodesiense</span></i><span style=\"font-family:Verdana;\"> HAT
serodiagnostic tests.
%K Human African Trypanosomiasis
%K TbgI&lt
%K sub&gt
%K 2&lt
%K /sub&gt
%K TbgI&lt
%K sub&gt
%K 17&lt
%K /sub&gt
%K Antigens
%K Diagnosis
%K &lt
%K i&gt
%K Trypanosoma brucei rhodesiense&lt
%K /i&gt
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=96585