%0 Journal Article
%T A UHPLC¨CMS/MS method for the quantification of ¦¤9-tetrahydrocannabinol and cannabidiol in decoctions and in plasma samples for therapeutic monitoring of medical cannabis | Bioanalysis
%A Antonietta Martelli
%A Carmen Fucile£¿
%A Elisa De Gr
%A Francesca Mattioli
%A Gino Tripodi
%A Giuliana Cangemi
%A Luca Manfredini
%A Marcella Gherzi
%A Sebastiano Barco£¿
%A is
%J Bioanalysis
%D 2018
%R https://doi.org/10.4155/bio-2018-0184
%X Aim: Monitoring of blood levels of ¦¤9-tetrahydrocannabinol (THC) and cannabidiol (CBD) is necessary for optimization of administration of medical cannabis. We describe the validation of a ultra-HPLC¨CMS/MS method for quantifying THC and CBD from plasma and decoctions and its application for therapeutic drug monitoring.Materials & methods: Analyses were performed by using a TSQ Quantiva£¿ Triple Quadrupole coupled to a Ultimate 3000 UHPLC system with atmospheric pressure chemical ionization after sample preparation with a straightforward method with deuterated internal standards. Results: The method has been validated following EMA guidelines and is linear in plasma from 0.16 to 10 ng/ml for both THC and CBD and in decoctions from 4.7 to 600 ng/ml. Conclusion: Given the unpredictable pharmacokinetic behavior of THC and CBD in patients, monitoring of plasma concentrations is strongly recommended for patients under treatment with medical cannabis.
%U https://www.future-science.com/doi/10.4155/bio-2018-0184